Abstract
In microCT imaging, there is a close relationship between the dose of radiation absorbed by animals and the image quality, or spatial resolution. Although the radiation levels used in these systems are generally non-lethal, they can induce cellular or molecular alterations that affect the experimental results. Here, we describe a dosimetric characterization of the different image acquisition modalities used by the microCT unit of the Albira microPET/SPECT/CT scanner, which is a widely used multimodal imaging system in preclinical research. The imparted dose at the animal surface (IDS) was estimated based on Boone’s polynomial interpolation method and experimental measurements using an ionization chamber and thermoluminescent dosimeters. The results indicated that the imparted dose at surface level delivered to the mice was in the 30 to 300 mGy range. For any combination of current (0.2 or 0.4 mA) and voltage (35 or 45 kV), in the Standard, Good, and Best image acquisition modalities, the dose imparted at surface level in rodents was below its threshold of deterministic effects (250 mGy); however, the High Res modality was above that threshold.
Highlights
MicroCT systems used in preclinical research as non-invasive systems allow the threedimensional imaging of small animals in order to evaluate human disease models [1]
In microCT imaging, there is a close relationship between the dose of radiation absorbed by animals and the image quality, or spatial resolution, and contrast [3,4,5]
Mainly based on single-photon emission computed tomography (SPECT), positron emission tomography (PET), and CT imaging techniques, have been developed and made commercially available for the performance of research and have the potential to be applied in a clinical setting
Summary
MicroCT systems used in preclinical research as non-invasive systems allow the threedimensional imaging of small animals (typically mice and rats) in order to evaluate human disease models [1]. These systems operate under the same principle as a CT system for clinical use, i.e., the three-dimensional reconstruction of a set of two-dimensional images (the projections) of the body’s cross-section, around which the system rotates [2]. Mainly based on SPECT, PET, and CT imaging techniques, have been developed and made commercially available for the performance of research and have the potential to be applied in a clinical setting In these systems, microCT has been used to provide morphological information for the obtainment of the spatial localization of the radiotracer distribution within the body. A critical concern in these multimodal imaging systems is the ionizing radiation dose received by the animals
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