Abstract

Objective To establish the analysis criteria of nucleoplasmic bridges (NPB) and a dose-response curve of NPB frequencies in human peripheral blood lymphocytes irradiated by 60Coy-rays. Methods Human peripheral blood samples were collected from three healthy males, and were irradiated with O, 1, 2, 3, 4, 5 and 6 Gy of 60 Co y-rays. A cytokinesis-block micronucleus assay was carried out to analyze NPBs and micronuclei (MN) in binucleated cells. Results NPBs in binucleated ceils at each dose level of ,/-ray was conformed to the Poisson distribution. The dose-response curve of the y-ray-induced NPB frequencies in human peripheral lymphocytes followed the linear-quadratic model y = ( 1.39 × 10 -3 )x2 + (4.94× 10-3)x (R2 =0.981, P 〈 0.05). Conclusions The dose-response curve of NPB frequencies in human peripheral blood lymphocytes induced by 0 -6 Gy 60Co y-rays was established. Key words: Ionizing radiation; Nucleoplasmic bridge; Micronucleus; Dose response

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