Dose-Response Curve of Chromosome Aberrations in Human Lymphocytes Induced by Gamma-Rays

Abstract

Chromosome aberration is a biomarker to predict the level of cell damage caused by exposure to ionizing radiation on human body . Dicentric chromosome is a specific chromosome aberration caused by ionizing radiation and is used as a gold standard biodosimetry of individuals over exposed to ionizing radiation . In radiation accident the dicentric assays has bee n applied as biological dosimetry to estimat e radiation absorbed dose and also to confirm the radiation dose received to radiation workers. The purpose of this study was to generate a dose response curve of chromosome aberration (dicentric) in human lymphocyte induced by gamma radiation . Peripheral blood samples from three non smoking healthy volunteers aged between 25-48 year s old with informed consent were i rradiat ed with dose between 0.1 - 4.0 Gy and a control using gamma teletherapy source . The culture procedure was conducted following the IAEA standard procedures with slight modifications. Analysis of dose-response curves used was LQ model Y = a + αD + βD 2 . The result show ed that α and β values of the curve obtained were 0.018 ± 0.006 and 0.013 ± 0.002, respectively. Dose response calibration curve for dicentric chromosome aberrations in human lymphocytes induced b y gamma -ra diation fitted to linear quadratic model. I n order to apply the dose response curve of chromosome aberration disentric for biodosimetry, this standar curve still need to be validated Received: 8 November 2013; Revised: 27 December 2013; Accepted: 31 December 2013