Dose-dependent effects of probiotic supplementation on bone characteristics and mineralisation in meat-type female turkeys

Abstract

To evaluate the influence of the probiotic on bone tissue in female turkeys, bone mineral density and geometrical and mechanical properties of the tibia and femur were determined in a dose-dependent manner (107 colony-forming units (cfu)/g, 108 cfu/g, 109 cfu/g). No effect of the treatments on bone mass and wall thickness of femur was observed, but the administration of the probiotic resulted in the elongation and the reduction of both strengths. The increase in the cross-sectional area of the femur was dose-dependent. Probiotic supplementation at a concentration of 108 cfu/g resulted in a reduction in ultimate strength, but at a concentration of 107 cfu/g, it resulted in the enhancement of the maximum elastic strength of the tibia compared with other groups. The influence of the probiotic administration on tibia geometry was dose-dependent. No effect of the treatments on the relative bone weight and the ratio of mass to length was observed. In general, the influence of the probiotic administration on bone mineral density, bone mineral concentration, bone tissue density, and bone ash, calcium and phosphorus concentrations was dose-dependent. The investigated properties of long bones in female turkeys are affected through probiotic-supplemented diets in a dose-dependent manner. However, on the basis of densitometry, it seems that the administration of the probiotic at a higher concentration of cells is more beneficial for bone development in turkeys.