Dose-dependent effect of Dendrobium officinale polysaccharide on anti-aging in Caenorhabditis elegans: A metabolomics analysis focused on lipid and nucleotide metabolism regulation

Abstract

Dendrobium officinale polysaccharide (DOP) has garnered attention for its potential anti-aging effects, yet the dose-dependent impact on aging, especially from a metabolomics perspective, remains less explored. Here, we investigated the dose-dependent effect of DOP on anti-aging in Caenorhabditis elegans, examining underlying mechanisms through metabolomics analysis. Based on food clearance assays, doses of 62.5, 125.0, and 250.0 mg/L of DOP were selected for lifespan experiments in C. elegans. Results indicated that both 125 and 250 mg/L DOP significantly extended lifespan, enhanced stress resistance, and preserved locomotion behavior, with superior effects observed at 250 mg/L. Metabolomics analysis revealed a dose-effect relationship between DOP concentration and 14 metabolites, primarily associated with lipid and nucleotide metabolism. Notably, metabolites including docosapentaenoic acid (DPA), arachidonic acid, uric acid, uridine 5′-diphosphogalactose (UDP-galactose), UDP-N-acetylglucosamine (UDP-GlcNAc), citric acid, l-Saccharopine, L-2-Aminoadipic acid, 5′-S-Methyl-5′-thioadenosine, 1-Linoleoyl glycerol, cyclic ADP-ribose, glycerol 3-phosphate (G3P), and adenosine exhibited increased levels with higher DOP doses, while palmitic acid levels declined with escalating DOP dose in the range from 62.5 to 250.0 mg/L. Furthermore, Pearson correlation analysis revealed strong associations between mean lifespan and levels of DPA, palmitic acid, G3P, UDP-galactose and UDP-GlcNAc. These findings underscore a significant relationship between lifespan extension and metabolic products of lipid and nucleotide metabolism in DOP-treated C. elegans, shedding light on potential mechanisms underlying anti-aging effects of DOP.