Dosage Effect of Zinc Glycine Chelate on Zinc Metabolism and Gene Expression of Zinc Transporter in Intestinal Segments on Rat.

Abstract

Zinc plays an essential role in various fundamental biological processes. The focus of this research was to investigate the dosage effect of zinc glycine chelate (Zn-Gly) on zinc metabolism and the gene expression of zinc transporters in intestinal segments. A total of 30 4-week-old SD rats were randomized into five treatment groups. The basal diets for each group were supplemented with gradient levels of Zn (0, 30, 60, 90, and 180mg/kg) from Zn-Gly. After 1-week experiment, the results showed that serum and hepatic zinc concentration were elevated linearly with supplemental Zn levels from 0 to 180mg Zn/kg. Serum Cu-Zn SOD activities resulted in a significant (P<0.01) quadratic response and reached the peak when fed 60mg Zn/kg. There were linear responses to the addition of Zn-Gly from 0 to 180mg Zn/kg on Cu-Zn SOD and AKP activities in the liver. In the duodenum, MT1 mRNA was upregulated with the increasing dietary Zn-Gly levels and reached the peak of 180mg Zn/kg (P<0.05). Zip4 mRNA expression was downregulated with the increasing zinc levels (P<0.05) in both duodenum and jejunum. In the jejunum, Zip5 mRNA expression in 60mg Zn/kg was higher compared with other groups (P<0.05). ZnT1 mRNA in duodenum was numerically increased with the rising levels of zinc content and was significantly higher (P<0.05) with 180mg Zn/kg. In the duodenum, adding 60 or 90mg Zn/kg increased PepT1 expression, but in the jejunum, 60mg Zn/kg did not differ from 0 added Zn. In summary, there is a dose-dependent effect of dietary Zn-Gly on serum and hepatic zinc levels and the activities of Cu-Zn SOD and AKP on rats. Dietary Zn-Gly has a certain effect on MT1, Zip4, Zip5, and ZnT1 expression, which expressed differently in intestinal segments with different levels of Zn-Gly load. Besides, Zn-Gly also could regulate PepT1 expression in intestinal segments.