Abstract
Comparison of three methods: the gravimetric method, that of Karl Fisher and gas chromatography for the calculation of residual humidity of vaccines, proves that their results are not interchangeable; and that, in particular, the gravimetric method produces higher titres than the other two. The gas chromatography method shows slightly lower results than the Karl Fisher method and the facility of repeating the observations is just as valid as long as one does not neglect to examine many samples since it is possible to carry out lots of measurements on each individual sample. Gas chromatography remains a difficult method which, in the end, does not present any decisive advantages over the Karl Fisher method. The calculations of residual humidity is a method by which one can check the accuracy of the procedures in manufacturing lyophilized vaccines; under no circumstances may it replace the biological test of accelerated preservation which is the only method which enables one to check the viability of the elements of the vaccine as we will show when discussing measles vaccine.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
