Abstract
Summary Homocysteine (Hcy) is a thiol containing amino acid related to methionine demethylation. Increased Hcy concentration is considered as a risk factor for cardiovascular diseases. In clinical laboratories, Hcy measurement can be performed using diverse methods such as chromatography, immunonephelemetry, immunochimiluminescence or enzymatic colorimetry. The goal of this work was to correlate two methods: high-pressure liquid chromatography (HPLC) versus immunonephelemetry. We studied 196 plasmatic samples collected in two hospitals, according to established protocols. The statistical analysis showed strong correlation and concordance between the two techniques. Hcy measurements using immunonephelemetry are underestimated by 25% compared to HPLC, leading to normality and cut-off changes for immunonephelemetry to 12 μM compared to 15 μM for HPLC. Using this value, sensibility of the measurement is 82% and specificity is 94.5%. In these conditions, Hcy measurements in immunonephelemetry on BN ProSpec (Siemens ® ) are as dependable as those of HPLC and applicable, as it is currently the case in CHU of Saint-Etienne.
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