DORN1/P2K1 and purino-calcium signalling in plants: making waves with extracellular ATP

Elsa Matthus,Limin Wang,Valérie Legué,Nathalie Leblanc-Fournier,Amirah B Mohammad-Sidik,Jian Sun,Katie A Wilkins,Bruno Moulia,Gary Stacey,Madhura G Bhat,Julia M Davies

doi:10.1093/aob/mcz135

Abstract

Background and AimsExtracellular ATP governs a range of plant functions, including cell viability, adaptation and cross-kingdom interactions. Key functions of extracellular ATP in leaves and roots may involve an increase in cytosolic free calcium as a second messenger (‘calcium signature’). The main aim here was to determine to what extent leaf and root calcium responses require the DORN1/P2K1 extracellular ATP receptor in Arabidopsis thaliana. The second aim was to test whether extracellular ATP can generate a calcium wave in the root.MethodsLeaf and root responses to extracellular ATP were reviewed for their possible links to calcium signalling and DORN1/P2K1. Leaves and roots of wild type and dorn1 plants were tested for cytosolic calcium increase in response to ATP, using aequorin. The spatial abundance of DORN1/P2K1 in the root was estimated using green fluorescent protein. Wild type roots expressing GCaMP3 were used to determine the spatial variation of cytosolic calcium increase in response to extracellular ATP.Key ResultsLeaf and root ATP-induced calcium signatures differed markedly. The leaf signature was only partially dependent on DORN1/P2K1, while the root signature was fully dependent. The distribution of DORN1/P2K1 in the root supports a key role in the generation of the apical calcium signature. Root apical and sub-apical calcium signatures may operate independently of each other but an apical calcium increase can drive a sub-apical increase, consistent with a calcium wave.ConclusionDORN1 could underpin several calcium-related responses but it may not be the only receptor for extracellular ATP in Arabidopsis. The root has the capacity for a calcium wave, triggered by extracellular ATP at the apex.

Highlights

adenosine 5′-triphosphate disodium salt trihydrate (ATP) is the universal cellular energy currency
Leaf and root [Ca2+]cyt signatures differ The findings presented here clearly show that Arabidopsis leaf and root extracellular ATP (eATP)-induced [Ca2+]cyt signatures differ in their time courses and amplitude
DORN1 still provides an important experimental gateway into the further dissection of purine–calcium signalling in roots and leaves

Summary

Introduction

ATP is the universal cellular energy currency. In plant cells, cytosolic ATP occurs at 0.5–2 mm (Gout et al, 2014; De Col et al, 2017). Whilst the mechanisms of ATP release into the plant cell’s extracellular space remain under investigation (Kim et al, 2006; Song et al, 2006; Rieder and Neuhaus, 2011; Wu et al, 2011), accumulation of extracellular ATP (eATP) to nanomolar levels occurs during growth and possibly to higher levels in response to abiotic and biotic stimuli (Jeter et al, 2004; Kim et al, 2006; Song et al, 2006; Weerasinghe et al, 2009; Clark et al, 2010; Dark et al, 2011; Zhu et al, 2017; Nizam et al, 2019). EATP has the hallmarks of a tightly regulated plant cell regulator

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