Dormancy-breaking and germination requirements for seeds ofDiervilla lonicera(Caprifoliaceae), a species with underdeveloped linear embryos

Abstract

Dormancy-breaking requirements and type of dormancy were determined for seeds of Diervilla lonicera Mill. Seeds have an underdeveloped linear embryo that is about 35% of the length of the seed at maturity. Embryos (in intact seeds) grew at 25:15°C but not at 5°C. Up to 85% of the freshly matured seeds had morphological dormancy (MD), and thus, they germinated within about 30 days on a moist substrate in light at 30:15°C; a maximum of 3% of the seeds germinated in constant darkness. The other portion of fresh seeds had nondeep simple morphophysiological dormancy (MPD) and required a period of warm stratification or treatment with GA3to break dormancy. These seeds also required light to germinate. In contrast, cold stratification induced dormancy, and dry storage for up to 1 year did not effectively break dormancy. Seeds with MD germinated to significantly higher percentages on soil than on filter paper or on sand. Seeds sown on soil in a non-temperature-controlled greenhouse in mid-November germinated mostly in late May, whereas those sown in mid-April germinated in early May. Apparently, embryos of November-sown seeds were induced into physiological dormancy during winter. Thus, seeds had MPD in spring and needed several weeks of warm temperatures for dormancy break, embryo growth, and germination. This is the first report on seed dormancy in the genus Diervilla.Key words: embryo growth, germination phenology, Diervilla lonicera, morphological seed dormancy, morphophysiological seed dormancy, underdeveloped linear embryo.