Abstract
We have examined the regulation of striatal GABA release by endogenous dopamine in rats with partial degeneration of dopamine-containing neurons. 6-Hydroxydopamine was administered into the lateral ventricles or medial forebrain bundle. Either 3 days or 3 weeks later, slices of neostriatum were prepared, preloaded with [ 3H]GABA, and superfused in order to measure [ 3H]GABA overflow in response to electrical stimulation (8 Hz). The loss of dopaminergic terminals was estimated by measuring tissue levels of dopamine. The impact of endogenous dopamine on [ 3H]GABA was evaluated by measuring the ability of sulpiride, a D 2 dopamine receptor antagonist, to increase the depolarization-induced [ 3H]GABA overflow. In non-treated or vehicle-pretreated rat neostriatum, sulpiride (10 μM) increased the depolarization-induced [ 3H]GABA overflow to 193% of control. Three days after lesioning, the stimulatory effect of sulpiride on [ 3H]GABA overflow was identical to that seen in control rats so long as the loss of tissue dopamine did not exceed 60%, although with larger lesions the sulpiride-induced response was reduced. Three weeks after lesioning, however, the stimulatory effect of sulpiride on electrically evoked [ 3H]GABA overflow remained at the level seen in control tissue even in cases where tissue dopamine was reduced to 13% of normal. In contrast, no sulpiride-induced increase in [ 3H]GABA overflow was detected 3 weeks after nearly complete lesions which reduced tissue dopamine to 2% of normal. These data suggest that short- and long-term compensatory changes maintain dopaminergic control over GABAergic projection neurons and interneurons until the loss of dopamine innervation is almost complete.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.