Abstract
Colocalization of proenkephalin and prodynorphin mRNAs with each other as well as with D1, D2, and D3 dopamine receptor mRNAs was analyzed in the nucleus accumbens of the rat. Distinct combinations were detected in the rostral pole, core, and shell subdivisions of the nucleus accumbens. Proenkephalin and prodynorphin mRNAs were principally localized in separate cells in the core. All detectable prodynorphin cells in the core expressed D1 mRNA but not D2 mRNA. Conversely, approximately 95% of the proenkephalin-positive cells in this region expressed D2 mRNA but not D1 mRNA. This pattern was identical to that observed in the caudate putamen. In the rostral pole and the shell, embedded in a background of this "typical" colocalization pattern, clusters of cells expressing a distinct configuration were found. In these clusters, proenkephalin-positive cells expressed both prodynorphin and D1 mRNAs, but they did not express D2 mRNA. D3 and prodynorphin mRNAs were colocalized in "limbic" striatal areas, including the ventromedial caudate putamen, the rostral pole, and the medial shell. In contrast, D3 mRNA was not detected in any proenkephalin-positive cells. Together with the prodynorphin/D1 data, this suggests that a subset of prodynorphin cells expresses both D1 and D3 mRNAs. It is concluded that 1) clusters of cells that coexpress proenkephalin, prodynorphin, and D1 mRNAs overlap extensively with previously defined cytoarchitectural cell clusters in the nucleus accumbens and 2) a subset of the prodynorphin cells in the ventromedial caudate putamen and the nucleus accumbens contains both D1 and D3 mRNAs.
Highlights
Colocalization of proenkephalin and prodynorphin mRNAs with each other as well as with D1, D2, and D3 dopamine receptor mRNAs was analyzed in the nucleus accumbens of the rat
Proenkephalin, D1 receptor, and D2 receptor signals were homogeneous within the caudate putamen (CPu), but prodynorphin signal was present in a patchy pattern
Within the nucleus accumbens (NAcc), the distribution patterns for all four of these messages were more complex, with areas of high signal intensity standing out against areas of low or no signal. This was especially clear in more rostral sections, where proenkephalin, prodynorphin, and D1 receptor signals were high in the ventral NAcc, but D2 receptor signal was absent
Summary
Colocalization of proenkephalin and prodynorphin mRNAs with each other as well as with D1, D2, and D3 dopamine receptor mRNAs was analyzed in the nucleus accumbens of the rat. Approximately 95%of the proenkephalin-positive cells in this region expressed D2 mRNA but not D1 mRNA This pattern was identical to that observed in the caudate putamen. In the rostral pole and the shell, embedded in a background of this “typical” colocalization pattern, clusters of cells expressing a distinct configuration were found. In these clusters, proenkephalin-positive cells expressed both prodynorphin and D1 mRNAs, but they did not express D2 mRNA. D3 and prodynorphin mRNAs were colocalized in “limbic” striatal areas, including the ventromedial caudate putamen, the rostral pole, and the medial shell. Comparisons of the parallel outputs of the NAcc to the ventral pallidum (VP)and the ventral tegmental area (VTA) are not as clear, but immunohistochemical studies suggest that the NAcc-VTA pathway may be similar to the striatonigral pathway, because there are large numbers of DYN-positive fibers within the VTA with far fewer
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