Dopamine Alleviates Hydrocarbon Stress in Brassica oleracea through Modulation of Physio-Biochemical Attributes and Antioxidant Defense Systems

Abstract

Hydrocarbon stress has become one of the most restrictive factors for crop choice and productivity in most parts of the world. Dopamine (DA) has positively influenced the metabolic, physiological and biochemical activities besides the growth of plants under numerous abiotic stress conditions. The current study was performed to analyze the potential of DA to alleviate hydrocarbon stress and improve growth of Brassica oleracea plants. Hydrocarbon stress in plants was induced by growing in 5% and 10% crude oil contaminated soil. Crude oil stressed plants exhibited reduced growth besides decreased level of photosynthetic pigments and gas exchange attributes. Moreover, oil stressed plants showed elevated level of hydrogen peroxide (H2O2), electrolyte leakage (EL), malondialdehyde (MDA) and superoxide radical (O2−). However, exogenous application of 50, 100 and 200 μmol L−1 DA improved photosynthesis, shoot and root dry weight of B. oleracea seedlings growing in hydrocarbon amended soil. Additionally, DA100 treatments improved non-enzymatic and enzymatic antioxidants of treated seedlings. Our results demonstrate that increased gas exchange attributes, modulation of osmoregulators and improved activity of the antioxidative enzymes alleviated hydrocarbon stress in DA supplemented B. oleracea plants. Consequently, the first time observed ameliorative role of DA in hydrocarbon stress opens a new arena for application of this dynamic biomolecule for sustainable crop production.