Abstract
γδ T lymphocytes play an important role in immune reactions towards infections and malignancies. In particular, Vγ9–Vδ1+ T lymphocytes are thought to play protective antiviral roles in human CMV infection. Recently, Vδ1+ T lymphocytes were proposed to also have anti- B-CLL reactivity. Here we report a case of 48-year-old man who received allogeneic stem cell transplantation for progressive B-CLL. Within one year after transplantation, lymphoma relapsed despite a dramatic increase of Vδ1+ T cells in the patient’s blood. In vitro killing assays revealed activity of patient’s γδ cells against CMV target cells, but not against the relapsing lymphoma-cells. This argues for a contribution of Vδ1+ cells in the immune reaction against CMV reactivation, but does not support a strong correlation of expanded Vδ1+ T cells and favorable disease outcome in B-CLL patients.
Highlights
Several types of T lymphocytes bearing γδ T cell receptors (TCR) are currently investigated as potential anticancer agents in cell-based immunotherapy
Activated Vδ1+ T lymphocytes can produce inflammatory cytokines such as TNF-α and IFN-γ and can be cytotoxic. Both cytokine release and cytotoxicity of Vδ1+ T lymphocytes are at least in part mediated through NKG2D receptor, which recognizes the stress-induced ligands MIC-A, MIC-B, and ULBP3 on target cells
Release cytotoxicity assay was performed by using sorted γδ T cells as effectors and either (A) CMV-infected primary human foreskin fibroblasts or (B) autologous tumor cells as targets
Summary
Immo Prinz1*, Kristina Thamm, Matthias Port, Eva M Weissinger, Michael Stadler, Ildar Gabaev, Roland Jacobs, Arnold Ganser and Christian Koenecke1,2*. Abstract γδ T lymphocytes play an important role in immune reactions towards infections and malignancies. Vγ9–Vδ1+ T lymphocytes are thought to play protective antiviral roles in human CMV infection. Within one year after transplantation, lymphoma relapsed despite a dramatic increase of Vδ1+ T cells in the patient’s blood. In vitro killing assays revealed activity of patient’s γδ cells against CMV target cells, but not against the relapsing lymphoma-cells. This argues for a contribution of Vδ1+ cells in the immune reaction against CMV reactivation, but does not support a strong correlation of expanded Vδ1+ T cells and favorable disease outcome in B-CLL patients
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