Abstract
Methods and Materials: 10 non-mobilized allogeneic donors have had DLI apheresis collections on Optia MNC version 5. Collection preference was set to deliver a low haematocrit final product. ACD-A anticoagulation was used and apheresis completed at an AC ratio of 13.5 and infusion rate of 0.8mL/min/L total blood volume (TBV). Run data were recorded collected from the Spectra Optia apheresis reporting system at the end of each procedure. Full blood count data and CD3+/CD19+ target cell numbers were completed for the donor before and after apheresis and from the final product. Performance was measured by target cell yield and collection efficiency together with final product volume and haematocrit. Platelet loss to the donor was also measured after apheresis.
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