Abstract

The nature of complex formation between aromatic amino acids and some of their components with NAD+ (nicotinamide adenine dinucleotide) have been studied by spectrophotometric methods. A comparison of the stability of these complexes with that of the complexes of the same donors with a model compound of NAD+i.e. N-methyl-3-carboxamide pyridinium chloride (MCP) has been carried out by examining the type of complexes formed and their geometry. Simple molecular orbital calculations have been done to determine the quantity of charge transfer δQ and stabilization energy δE and to show the significance of mutual orientation of donor and acceptor in determining the stability. The enhanced stability of tryptophan–NAD+ complex in comparison to indole–NAD+ complex has also been explained.

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