Abstract
BackgroundHumoral immunity plays an important role in the prevention of canine distemper. Anti-CD virus (CDV) antibody has strong antiviral activity and is widely used in the treatment of CD. However, with the increase of CD cases, the availability of therapeutic CD antibody fell short of the clinical needs.ResultsThe high-titer antiserum with the high-titer neutralizing activity against CDV was obtained from the donkeys (Dezhou Donkey) immunized with the inactivated CDV vaccine. The donkey anti-CDV IgG was purified from the donkey serum, which was identified to significantly inhibit the CDV replication in the cultured Vero cells and effectively reduce the clinical symptoms and increase the survival rates (75%) of CDV-infected dogs (Shih-tzu Dog), similar to that treated with the dog-derived anti-CDV IgG. These results indicate that donkey-derived IgG is a potential substitute for dog-derived IgG to treat the CD in clinic.ConclusionsAdministration of donkey-derived anti-CDV IgG can ameliorate clinical symptoms and inhibit virus replication, thereby increasing the survival of CDV-infected dogs. This study opens up a new source of therapeutic antibody for CD treatment.
Highlights
Humoral immunity plays an important role in the prevention of canine distemper
CD virus (CDV) vaccine To quantify the humoral immune response against CDV, donkeys were inoculated with inactivated CDV vaccine and the titers of CDV-specific antibodies in the serum of donkeys were measured by enzyme-linked immunosorbent assay (ELISA) at different times after immunization (Fig. 1a)
We found that CDV-specific antibodies emerges at 7 d after immunization in all donkey sera
Summary
Anti-CD virus (CDV) antibody has strong antiviral activity and is widely used in the treatment of CD. With the increase of CD cases, the availability of therapeutic CD antibody fell short of the clinical needs. Canine distemper (CD) is an acute, highly contagious disease caused by canine distemper virus (CDV) in canines, clinically characterized by increased biphasic body temperature, subsequent bronchitis, catarrhal pneumonia, severe gastroenteritis and neurological symptoms. CDV has a wide range of hosts, infecting dogs of all ages and ferrets, raccoons and giant animals of many species, such as giant pandas, tigers, lions, pandas, lynx and bears [6, 7]. Effective prevention and treatment of CD is urgent and of vital importance
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
