Dominant chemical compound of chicken bile extract

Abstract

The purpose of this research is to study the dominant chemical compounds of chicken bile extract. Chicken bile, which is usually disposed of as useless waste, is made a choice. The study began with material collection from native chicken farmers, Kluwut Village, Bulakambah sub-district, Brebes Regency, Central Java. The determination was carried out at Bandungense Herbarium School of Biological Science and Technology-ITB. The choice of animals were native chickens. They belonged to the family Phylum: Chordata, Class: Aves, Nation: Galliformes, Tribe: Phasianidae, Surname: Gallus, Type: Gallus gallus Linn, The common name: native chicken (Indonesia), and domestic fowl in English. The bile portion was cut into small pieces and dried using a freeze dryer. The reflux method was then extracted using solvents with a different polarity, which are n-hexane, ethyl acetate, and ethanol. It gave out n-hexane extract, ethyl acetate extract, and ethanol extract. The extracts were evaporated using a rotary evaporator. The percentage of the obtained yield was n-hexane extract 7.63%, ethyl acetate extract 8.61%, and ethanol extract 34.91%. Selected ethyl acetate extract was fractionated by Vacuum Liquid Chromatography (VLC I) and was monitored by a thin layer of Chromatography (TLC). Then fraction 5-6 was continued to sub fractionation by Classical Column Chromatography (CCC). Nuclear Magnetic Resonance (NMR) investigated isolate X and found that isolate X appeared to be cholesterol. From the second fractionation (VLC II), fraction 2-7 proceeded to the VLC III, and then subfraction 7 was evaluated using GC-MS. The dominant chemical compounds of subfraction 7 were oleic acid 38.72%, n-hexadecanoic acid (35.6%), octadecanoic acid (17.94%), palmitoleic acid (1.53%).