Dominant and Recessive Mutations Conferring Resistance to S-2-aminoethyl-L-cysteine in Maize

Abstract

Summary Maize tissue culture was use to select plants resistant to the lysine analogue, S-2-aminoethyl-L-cYsteine (AEC). Embryogenic calli of the maize ({itZea mays} L.) inbred me Cat-100-1 were mutagenize with NaN3 and selected in medium containing 0.25 mM AEC. Several plants were regenerated, transferred to soil and grown to maturity. Two plants, AEC-1 an AEC-5, 0 63 regenerated plants, when self-pollinated, segregate resistant and sensitive pants. The resistance of the AEC-1 plant proved to be determined by a single dominant gene while the resistance of AEC-5 was determined by a single recessive gene after the analysis of R 1 progenies obtained from AEC-1 and AEC-5 regenerated plants. Soluble amino acids from the endosperm of both mutants were extracted an quantified by high performance liquid chromatography. Both resistant mutants presented an increase soluble lysine concentration when compare with sensitive ones.