Dominance of bacterial ammonium oxidizers and fungal denitrifiers in the complex nitrogen cycle pathways related to nitrous oxide emission

Késia S Lourenço,Johannes A Van Veen,Eiko E Kuramae,Maurício R Dimitrov,Janaína B Do Carmo,Agata Pijl,Heitor Cantarella,Johnny R Soares

doi:10.1111/gcbb.12519

Abstract

AbstractOrganic compounds and mineral nitrogen (N) usually increase nitrous oxide (N2O) emissions. Vinasse, a by‐product of bio‐ethanol production that is rich in carbon, nitrogen, and potassium, is recycled in sugarcane fields as a bio‐fertilizer. Vinasse can contribute significantly to N2O emissions when applied with N in sugarcane plantations, a common practice. However, the biological processes involved in N2O emissions under this management practice are unknown. This study investigated the roles of nitrification and denitrification in N2O emissions from straw‐covered soils amended with different vinasses (CV: concentrated and V: nonconcentrated) before or at the same time as mineral fertilizers at different time points of the sugarcane cycle in two seasons. N2O emissions were evaluated for 90 days, the period that occurs most of the N2O emission from fertilizers; the microbial genes encoding enzymes involved in N2O production (archaeal and bacterial amoA, fungal and bacterial nirK, and bacterial nirS and nosZ), total bacteria, and total fungi were quantified by real‐time PCR. The application of CV and V in conjunction with mineral N resulted in higher N2O emissions than the application of N fertilizer alone. The strategy of vinasse application 30 days before mineral N reduced N2O emissions by 65% for CV, but not for V. Independent of rainy or dry season, the microbial processes were nitrification by ammonia‐oxidizing bacteria (AOB) and archaea and denitrification by bacteria and fungi. The contributions of each process differed and depended on soil moisture, soil pH, and N sources. We concluded that amoA‐AOB was the most important gene related to N2O emissions, which indicates that nitrification by AOB is the main microbial‐driven process linked to N2O emissions in tropical soil. Interestingly, fungal nirK was also significantly correlated with N2O emissions, suggesting that denitrification by fungi contributes to N2O emission in soils receiving straw and vinasse application.

Highlights

Vinasse is the major residue generated during ethanol production from sugarcane
The application of vinasse (CV and V) 30 days prior to mineral N fertilizer reduced the cumulative N2O emissions from straw-covered sugarcane fields by 65% and 37% compared to the application of vinasse and mineral N simultaneously in the first three months after vinasse application
The interval of 30 days between the application of vinasse and N fertilizer appears to be sufficient to ameliorate the anaerobic conditions induced by vinasse application and thereby decrease heterotrophic denitrification by nitrifiers and denitrifiers

Summary

Introduction

Vinasse is the major residue generated during ethanol production from sugarcane. For each liter of ethanol produced, approximately 10–15 L of vinasse are generated (Christofoletti, Escher, Correia, Marinho, & Fontanetti, 2013). In 2016, the annual production of vinasse was 360 billion liters in Brazil (CONAB, 2017). This immense volume of vinasse is difficult to manage for utilization as fertilizer. Concentration of vinasse by evaporation reduces the water content and the volume, providing an alternative residue with high nutrient and carbon contents (Christofoletti et al, 2013). Following evaporation, concentrated vinasse can be applied in the field, often in bands close to the plant row in a manner similar to that of mineral fertilizers, which reduces application cost and facilitates nutrient absorption by crops (Mutton, Rossetto, & Mutton, 2014; Parnaudeau, Condom, Oliver, Cazevieille, & Recous, 2008)

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