“Dome-curve” — three size classes of domes of MDCK epithelial monolayer

Abstract

Borders between monolayer of MDCK epithelial cells and domes were made visible by fixation and staining with 1% silver nitrate in 4% aqueous solution of mannitol. If the lengths of circumferences of domes are plotted against the frequency of their occurrence a "dome-curve" can be obtained. It was found that three size classes of domes exist in the MDCK monolayer: small with 0.0811 mm of circumference, medium with 0.1449 mm of circumference and large with 0.2439 mm of circumference.