Abstract
Five distinct serum amyloid A (SAA) cDNA clones have been isolated from a library constructed using hepatic mRNA isolated from an individual beagle dog with canine pain syndrome. This implies the existence of at least three SAA genes in the dog genome. One clone predicts a truncated "amyloid A-like" SAA molecule and offers a possible alternative mechanism for the pathogenesis of secondary amyloidosis. Relative to the human and mouse SAA proteins, an additional peptide of eight amino acids is specified by each of the dog cDNA clones. The existence of this peptide in all acute phase dog SAA proteins was confirmed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate of acute phase high density lipoprotein and provides supporting evidence for gene conversion as a mechanism for maintaining the homogeneity of the SAA gene family within a species. Analysis of hepatic RNA following induction of an acute phase response shows a dramatic increase in SAA mRNA concentration; the SAA transcripts show a transient increase in size early in inflammation due to an increase in polyadenylation.
Highlights
Five distinct serum amyloid A (SAA) cDNA clones characterized by the deposition of amyloid fibrils in anumber have been isolated from a library constructed using hepatic mRNA isolated from an individual beagle dog with canine pain syndrome
Analy- is not strictly conserved exhibit a decreased susceptibility to sis of hepatic RNA following induction of an acute secondary amyloidosis, i.e. rat hepatic SAA lacks this region phase response shows a dramatic increase in SAA mRNA concentration; the SAA transcripts show a transient increase in size early iinnflammation due to an increase in polyadenylation
The sequence reported for dog AA is 93 response to an inflammatory stimulus.SAA,’ a 12-kDa apo- residues long, 18 residues longer than human AA which has lipoprotein associated with HDL3, is the major acute phase 76, and shows 84% homology with human AA up to residue reactant in many species, including human and mouse, and 69
Summary
Five distinct serum amyloid A (SAA) cDNA clones characterized by the deposition of amyloid fibrils in anumber have been isolated from a library constructed using hepatic mRNA isolated from an individual beagle dog with canine pain syndrome.
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