Abstract
DNA binding with one finger (DOF) transcription factors play important roles in storage material accumulation and morphogenesis of developing seeds. Oil and protein contents varied in different cultivars in important oil crop peanut. DOF proteins have not been studied in this crop. In this paper, we analyzed all the DOF genes expressed in developing seeds from a cDNA library with 20,000 transcripts, cloned and compared similar genes of GW391729 from eight peanut cultivars, and analyzed similar genes expressed in root and leave with control and inoculated with Ralstonia solanacearum. The results indicate that total eight types of DOF genes were expressed in developing seeds of cultivar 063103. Most of DOF transcription factors expressed involved in developmental process in a complicated way. Among them, GW391729 is possible related to the seed number in fruit, and also is possible related to leafspot resistance. Detailed function of these DOF proteins need to be further studied.
Highlights
DNA binding with one finger (DOF) family proteins are plant specific with the structure feature of C2-C2 formed Zn-finger binding to DNA motif T/AAAAG at their N-terminal, which possess functions in various biological process in plants [1,2,3]
We analyzed all the DOF genes expressed in developing seeds from a cDNA library with 20,000 transcripts, cloned and compared similar genes of GW391729 from eight peanut cultivars, and analyzed similar genes expressed in root and leave with control and inoculated with Ralstonia solanacearum
DOF proteins have been classified into seven groups [1], and each group contains certain number of conserved sequences [2].The function of proteins are composed of subfunctions, which are determined by the responsive subdomains in the proteins
Summary
DOF family proteins are plant specific with the structure feature of C2-C2 formed Zn-finger binding to DNA motif T/AAAAG at their N-terminal, which possess functions in various biological process in plants [1,2,3]. The conserved DOF zinc finger domains in DOF proteins can bind to cis DNA core elements AAAG [6], and may interact with other proteins including DOF itself [7]. The differences in DOF zinc finger domains determined its binding affinity with specific cis AAAG motif, which could locate in different genes or in different elements of one promoter [8]. It is possible to bioinformatically analyze the function of unknown DOF proteins by paired comparison of similar amino acid sequences of conserved domains
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