Does vaccine-primed pancreatic cancer offer better candidates for immune-based therapies?

Abstract

ImmunotherapyVol. 6, No. 10 EditorialFree AccessDoes vaccine-primed pancreatic cancer offer better candidates for immune-based therapies?Lei ZhengLei ZhengE-mail Address: lzheng6@jhmi.eduDepartments of Oncology & Surgery, The Sidney Kimmel Cancer Center, & the Skip Viragh Center for Pancreatic Cancer Research & Clinical Care, Johns Hopkins University School of Medicine, 1650 Orleans Street, CRB1 Room 488, Baltimore, MD 21231, USASearch for more papers by this authorPublished Online:27 Nov 2014https://doi.org/10.2217/imt.14.87AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareShare onFacebookTwitterLinkedInRedditEmail Keywords: immunotherapypancreatic cancerPD-1PD-L1tertiary lymphoid aggregatesvaccineCancer immunotherapy is considered to be one of the biggest breakthroughs in cancer therapy in the last decade. However, the success of immunotherapy has so far been limited to a few solid malignancies including melanoma, renal cell carcinoma, non-small-cell lung cancer (NSCLC) and a few hematologic malignancies. In 2011, ipilimumab, a therapeutic monoclonal antibody that blocks CTLA-4, the bona fide immune checkpoint, was US FDA-approved for advanced melanoma [1]. Subsequently, other checkpoint inhibitors including anti-PD-1 and anti-PD-L1 blockade antibodies were also demonstrated to yield an objective response in approximately 20–30% of patients of these malignant diseases; and among the patients who had an objective response, many had a durable response [2–4]. One of anti-PD-1 antibodies (pembrolizumab) was most recently approved by US FDA for unresectable or metastatic melanoma. In addition, sipuleucel-T, a dendritic cell vaccine, has been shown to improve the overall survival of metastatic prostate cancer and subsequently gained FDA approval [5]. Nevertheless, significant objective response and durable responses were not seen in sipuleucel-T-treated pancreatic cancer patients. Melanoma, renal cell carcinoma and NSCLC were unique in their high infiltration of effector lymphocytes in tumor microenvironment (TME) [6]. By contrast, many other solid malignancies including pancreatic cancer are characterized by a highly immunosuppressive TME [7]. Immune tolerance mechanisms within the TME are a major obstacle to effective treatment of these cancers with immunotherapy. Pancreatic cancer and many other malignancies are thus considered 'nonimmunogenic' neoplasms. This notion has drastically slowed the development and application of immune-based therapies for these diseases.Immune tolerance mechanisms appear to have been established early in the development of pancreatic cancer [8]. At the stage of invasive adenocarcinoma, not only the TME is skewed toward immunosuppressive cells (e.g., M2 tumor-associated macrophage, myeloid-derived suppressive cells and T-regulatory cells), but the TME also lacks antigen experienced T-effector cells [7]. Several studies showed that pancreatic cancer TME is dominated by innate immune cells and lacks adaptive immune response [7,9]. Thus, the first barrier for an effective immunotherapy strategy to overcome is to activate the adaptive immune response in the TME. Vaccine-based immunotherapies are known to activate antigen-specific T-effector cells in the peripheral lymphocytes. However, it is not known whether vaccine-induced adaptive immune response occurs in the TME. We developed a GM-CSF-secreting pancreatic cancer vaccine, which has so far been one of the most widely tested immunotherapies for pancreatic cancer [10–13]. Our prior work demonstrated clinical activity in a subset of vaccinated patients, and showed that the post-vaccination induction of T-cell responses against mesothelin is associated with longer survival [11,12,14]. However, up to this point, neither our group nor other groups have had the opportunity to conduct in-depth analyses of the effects of an immune-based therapy on the TME of nonmelanoma solid tumors in patients.Our group has thus conducted a novel neoadjuvant and adjuvant study designed to evaluate postimmunotherapy changes within the TME of primary pancreatic tumors following treatment with our pancreatic cancer GM-CSF-secreting pancreatic cancer vaccine, given either alone or with immune modulating doses of cyclophosphamide to deplete regulatory T cells [15]. Given with the same pancreatic cancer vaccine, it was previously reported that low dose cyclophosphamide enhanced higher avidity T-cell responses that were associated with longer progression-free survival in patients [11]. Provided opportunities to dissect TME in the wholly resected tumors, the neoadjvuant vaccine study revealed novel findings that would not have been demonstrated in prior studies. Pathological examination of tumor tissue resected just 2 weeks following vaccination identified the formation of immunotherapy-induced tertiary lymphoid aggregates, an organized lymphoid structure that was not observed in tumors resected from unvaccinated patients.Tertiary lymphoid structures have not been previously reported in pancreatic cancer, but have been reported in other cancers at baseline, before therapeutic intervention [16]. Those cancers that demonstrated tertiary lymphoid aggregates at baseline include melanoma and NSCLC, which were also found to respond to the immune checkpoint inhibitors [2–4]. In contrast to primary and secondary lymphoid structure, tertiary lymphoid structures are not pre-existing and are developed only when challenged with antigens [16]. The development of tertiary lymphoid structures is achieved through a lymphoid neogenesis process by importing the lymphocytes from blood and lymph. Thus, the formation of tertiary lymphoid aggregates in pancreatic cancer in response to the vaccine therapy appears to be induced by adaptive immune response that is stimulated by antigens provided by the vaccine.Likely as a result of adaptive immune response to the vaccine treatment, PD-L1 expression is induced in the lymphoid aggregates by the pancreatic cancer vaccine treatment [15]. At baseline, a small percentage of pancreatic cancers express low levels of membranous PD-L1 on the epithelial tumor cells or stromal cells. By contrast, vaccine therapy induced the expression of membranous PD-L1 moderately on the epithelial tumor cells, but more significantly induced the infiltration of PD-L1 positive cells in all the intratumoral lymphoid aggregates [15]. PD-L1 expression may be regulated by oncogenic pathways. However, in most cancers, PD-L1 is induced by cytokines such as IFN-γ as a result of adaptive immune response [17]. In melanoma, NSCLC and renal cell carcinoma, PD-L1 expression was observed in tumor cells in approximately 53–89% of untreated patients’ tumors and in tumor infiltrating immune cells in approximately 50–100% of tumors [6]. PD-L1 expression in both tumor cells and tumor infiltrating immune cells is associated with PD-1 expression in tumor-infiltrating lymphocytes, which was also associated with more abundant infiltration of immune cells and the presence of lymphoid aggregates in these untreated patients’ tumors. The high prevalence of PD-L1 expression in these malignancies may explain their relatively high response rates to anti-PD-1 or anti-PD-L1 therapies [2–4]. By contrast, pancreatic cancer demonstrated a minimal response to anti-PD-1/PD-L1 therapies, which is likely due to low PD-L1 expression in pancreatic cancer. However, vaccine therapy, by inducing PD-L1 expression in TME, may have primed the pancreatic cancers for anti-PD-1/PD-L1 therapies.The combination of anti-PD-1/PD-L1 therapies and vaccine therapy was tested by our group in the preclinical model of pancreatic cancer [18]. Interestingly, anti-PD-1/PD-L1 therapies alone did not significantly alter the TME of murine pancreatic tumors. By contrast, vaccine therapies moderately enhanced the infiltration of effector T cells into TME. However, by combining with the vaccine therapies, antitumor effector T-cell response in the TME was significantly enhanced by anti-PD-1/PD-L1 therapies. As a result, the combination of vaccine and anti-PD-1/PD-L1 therapies has increased the cure rate of tumor-bearing mice compared with either single therapy alone. This yet unpublished follow-up study suggests that vaccine therapy is more critical in priming pancreatic cancer TME with effector T-cell infiltration, and that the combination of anti-PD-1/PD-L1 therapies, by overcoming adaptive immune resistance, is more critical in inducing an effective antitumor immune response. A clinical trial that is supported by this preclinical study will soon be initiated as part of the Stand Up To Cancer (SU2C) project.Besides anti-PD-1/PD-L1 therapies, vaccine therapy may prime pancreatic cancer for other immune-based therapies. Gene microarray analysis of microdissected lymphoid aggregates from the pancreatic cancer patients treated with the neoadjuvant vaccine therapy identified gene signatures in five pathways, including the TH17/T-regulatory cell, NF-κB, integrin/adhesion, chemokine/chemokine receptor and ubiquitin-proteasome pathways, which are associated with improved post-vaccination immune responses and longer survival [15]. Therefore, lymphoid aggregates can express both effector activating and effector downregulatory immune signatures. Thus, vaccine therapy potentially primes pancreatic cancer to other immune-based therapies or targeted therapies that enhance effector activating immune signatures or that inhibit effector downregulatory immune signatures.Vaccine therapy is obviously not the only therapy that can prime the cancer for immunotherapies. Epigenetic therapies including DNA demethylating agents or histone deacytelation inhibitors have also been suggested to prime NSCLC for anti-PD-1 therapies [19]. Although the exact mechanisms are unknown, a broad immune stimulatory role for DNA demethylating drugs in multiple cancers including colorectal cancer, breast cancer, ovarian cancer, NSCLC and a number of hematologic malignancies has been proposed [19,20]. DNA demethylating agents were found to induce the expression of cancer testis antigens that are not expressed in most of cancers at baseline and thus are not tolerated by the patients’ immune system. In addition, DNA demethylating treatments upregulate immunomodulatory pathways including interferon signaling, antigen presentation and certain cytokines/chemokines [19,20]. PD-L1 expression also appeared to be induced by DNA demethylating treatments [19]. Thus, clinical trials testing the role of epigenetic therapy in priming the cancer for anti-PD-1 therapies are being conducted. Nevertheless, other modalities of treatments that generate an inflammatory response may also have roles in priming the TME for immunotherapies. Moreover, one type of immunotherapy may also prime the TME for another type of immunotherapy. One good example is that the combinatorial therapy of anti-CTLA-4 antibody and anti-PD-1 antibody resulted in higher response rate and durable response. This concept has been tested in the preclinical model and subsequently supported by the clinical trial of the combination of ipilimumab and nivolumab for advanced melanomas [21]. Thus, even for those malignancies that are relatively sensitive to immune checkpoint inhibitors, the priming mechanism through a combinational immunotherapy approach would potentially convert those immunotherapy nonresponding patients to responders.In conclusion, vaccine therapy is able to reprogram the TME of pancreatic cancer and potentially that of many other malignancies. By reprogramming the TME, vaccine therapy may have primed these malignancies for more effective immunotherapies. Such a treatment strategy may also be applied to other combinational modalities of cancer therapies.Financial & competing interests disclosureL Zheng was supported by NIH K23 CA148964-01, Johns Hopkins School of Medicine Clinical Scientist Award, American Society of Clinical Oncology Young Investigator Award, Viragh Foundation and the Skip Viragh Pancreatic Cancer Center at Johns Hopkins, National Pancreas Foundation, Lefkofsky Family Foundation, NCI SPORE in Gastrointestinal Cancers P50 CA062924, and Lustgarten Foundation. The author has no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.No writing assistance was utilized in the production of this manuscript.References1 Hodi FS, O'Day SJ, McDermott DF et al. Improved survival with ipilimumab in patients with metastatic melanoma. N. Engl. J. Med. 363(8), 711–723 (2010).Crossref, Medline, CAS, Google Scholar2 Brahmer JR, Tykodi SS, Chow LQ et al. Safety and activity of anti-PD-L1 antibody in patients with advanced cancer. N. Engl. J. Med. 366(26), 2455–2465 (2012).Crossref, Medline, CAS, Google Scholar3 Topalian SL, Hodi FS, Brahmer JR et al. Safety, activity, and immune correlates of anti-PD-1 antibody in cancer. N. Engl. J. Med. 366(26), 2443–2454 (2012).Crossref, Medline, CAS, Google Scholar4 Hamid O, Robert C, Daud A et al. Safety and tumor responses with lambrolizumab (anti-PD-1) in melanoma. N. Engl. J. Med. 369(2), 134–144 (2013).Crossref, Medline, CAS, Google Scholar5 Kantoff PW, Higano CS, Shore ND et al. Sipuleucel-T immunotherapy for castration-resistant prostate cancer. N. Engl. J. Med. 363(5), 411–422 (2010).Crossref, Medline, CAS, Google Scholar6 Taube JM, Klein AP, Brahmer JR et al. Association of PD-1, PD-1 ligands, and other features of the tumor immune microenvironment with response to anti-PD-1 therapy. Clin. Cancer Res. 20(19), 5064–5074 2014).Crossref, Medline, CAS, Google Scholar7 Zheng L, Xue J, Jaffee EM, Habtezion A. Role of immune cells and immune-based therapies in pancreatitis and pancreatic ductal adenocarcinoma. Gastroenterology 144(6), 1230–1240 (2013).Crossref, Medline, Google Scholar8 Clark CE, Hingorani SR, Mick R, Combs C, Tuveson DA, Vonderheide RH. Dynamics of the immune reaction to pancreatic cancer from inception to invasion. Cancer Res. 67(19), 9518–9527 (2007).Crossref, Medline, CAS, Google Scholar9 Beatty GL, Chiorean EG, Fishman MP et al. CD40 agonists alter tumor stroma and show efficacy against pancreatic carcinoma in mice and humans. Science 331(6024), 1612–1616 (2011).Crossref, Medline, CAS, Google Scholar10 Jaffee EM, Hruban RH, Biedrzycki B et al. Novel allogeneic granulocyte-macrophage colony-stimulating factor-secreting tumor vaccine for pancreatic cancer: a Phase I trial of safety and immune activation. J. Clin. Oncol. 19(1), 145–156 (2001).Crossref, Medline, CAS, Google Scholar11 Laheru D, Lutz E, Burke J et al. Allogeneic granulocyte macrophage colony-stimulating factor-secreting tumor immunotherapy alone or in sequence with cyclophosphamide for metastatic pancreatic cancer: a pilot study of safety, feasibility, and immune activation. Clin. Cancer Res. 14(5), 1455–1463 (2008).Crossref, Medline, CAS, Google Scholar12 Lutz E, Yeo CJ, Lillemoe KD et al. A lethally irradiated allogeneic granulocyte-macrophage colony stimulating factor-secreting tumor vaccine for pancreatic adenocarcinoma. A Phase II trial of safety, efficacy, and immune activation. Ann. Surg. 253(2), 328–335 (2011).Crossref, Medline, Google Scholar13 Le DT, Lutz E, Uram JN et al. Evaluation of ipilimumab in combination with allogeneic pancreatic tumor cells transfected with a GM-CSF gene in previously treated pancreatic cancer. J. Immunother. 36(7), 382–389 (2013).Crossref, Medline, CAS, Google Scholar14 Thomas AM, Santarsiero LM, Lutz ER et al. Mesothelin-specific CD8(+) T cell responses provide evidence of in vivo cross-priming by antigen-presenting cells in vaccinated pancreatic cancer patients. J. Exp. Med. 200(3), 297–306 (2004).Crossref, Medline, CAS, Google Scholar15 Lutz ER, Wu AA, Bigelow E et al. Immunotherapy converts nonimmunogenic pancreatic tumors into immunogenic foci of immune regulation. Cancer Immunol. Res. 2(7), 616–631 (2014).Crossref, Medline, CAS, Google Scholar16 Goc J, Fridman WH, Sautes-Fridman C, Dieu-Nosjean MC. Characteristics of tertiary lymphoid structures in primary cancers. Oncoimmunology 2(12), e26836 (2013).Crossref, Medline, Google Scholar17 Taube JM, Anders RA, Young GD et al. Colocalization of inflammatory response with B7-h1 expression in human melanocytic lesions supports an adaptive resistance mechanism of immune escape. Sci. Transl. Med. 4(127), 127ra137 (2012).Crossref, Google Scholar18 Soares KC, Rucki AA, Wu A et al. PD-1/PD-L1 blockade together with vaccine therapy facilitates effector T cell infiltration into pancreatic tumors. J. Immunotherapy (2014) (In Press).Medline, Google Scholar19 Wrangle J, Wang W, Koch A et al. Alterations of immune response of non-small cell lung cancer with azacytidine. Oncotarget 4(11), 2067–2079 (2014).Crossref, Google Scholar20 Li H, Chiappinelli KB, Guzzetta AA et al. Immune regulation by low doses of the DNA methyltransferase inhibitor 5-azacitidine in common human epithelial cancers. Oncotarget 5(3), 587–598 (2014).Crossref, Medline, Google Scholar21 Wolchok JD, Kluger H, Callahan MK et al. Nivolumab plus ipilimumab in advanced melanoma. N. Engl. J. Med. 369(2), 122–133 (2013).Crossref, Medline, CAS, Google ScholarFiguresReferencesRelatedDetailsCited ByTargeting immune checkpoints: Building better therapeutic puzzle in pancreatic cancer combination therapy27 May 2020 | European Journal of Cancer Care, Vol. 29, No. 5Pancreatic ductal adenocarcinoma immune microenvironment and immunotherapy prospects1 March 2020 | Chronic Diseases and Translational Medicine, Vol. 6, No. 1IDO1 inhibition potentiates vaccine-induced immunity against pancreatic adenocarcinoma18 March 2019 | Journal of Clinical Investigation, Vol. 129, No. 4From immune checkpoints to vaccines: The past, present and future of cancer immunotherapyClinical evaluation of macrophages in cancer: role in treatment, modulation and challenges25 September 2017 | Cancer Immunology, Immunotherapy, Vol. 66, No. 12Overcoming the resistance of pancreatic cancer to immune checkpoint inhibitors19 June 2017 | Journal of Surgical Oncology, Vol. 116, No. 1Combinational Immunotherapy of Gastric Cancer16 May 2017 Vol. 6, No. 10 Follow us on social media for the latest updates Metrics History Published online 27 November 2014 Published in print October 2014 Information© Future Medicine LtdKeywordsimmunotherapypancreatic cancerPD-1PD-L1tertiary lymphoid aggregatesvaccineFinancial & competing interests disclosureL Zheng was supported by NIH K23 CA148964-01, Johns Hopkins School of Medicine Clinical Scientist Award, American Society of Clinical Oncology Young Investigator Award, Viragh Foundation and the Skip Viragh Pancreatic Cancer Center at Johns Hopkins, National Pancreas Foundation, Lefkofsky Family Foundation, NCI SPORE in Gastrointestinal Cancers P50 CA062924, and Lustgarten Foundation. The author has no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.No writing assistance was utilized in the production of this manuscript.PDF download