Abstract
Adipose-derived stem cells (ASCs) possess a high differentiation and proliferation potential. However, the phenotypic characterization of ASCs is still difficult. Until now, there is no extensive analysis of ASCs markers depending on different liposuction methods. Therefore, the aim of the present study was to analyse 242 surface markers and determine the differences in the phenotypic pattern between ASCs obtained during mechanical and ultrasound-assisted liposuction. ASCs were isolated from healthy donors, due to mechanical and ultrasound-assisted liposuction and cultured in standard medium to the second passage. Differentiation potential and markers expression was evaluated to confirm the mesenchymal nature of cells. Then, the BD LyoplateTM Human Cell Surface Marker Screening Panel was used. Results shown that both population of ASCs are characterized by high expression of markers specific for ASCs: cluster of differentiation (CD)9, CD10, CD34, CD44, CD49d, CD54, CD55, CD59, CD71 and low expression of CD11a, CD11c and CD144. Moreover, we have noticed significant differences in antigen expression in 58 markers from the 242 studied. Presented study shows for the first time that different liposuction methods are not a significant factor which can influence the expression of human ASCs surface markers.
Highlights
The first application of tissue engineering was based on the use of differentiated cells of the adult organism [1]
adipose-derived stem cell (ASC) purification has been performed by prolonged culture of SVF, relying on the ability of ASCs to outcompete other cell populations under the culture conditions over time
On the basis of researchers conducted so far, in June 2013, the International Society of Cell Therapy (ISCT) and the International Federation for Adipose Therapeutics and Science (IFATS) made a statement concerning the minimum criteria defining a fraction of SVF cells and ASCs
Summary
The first application of tissue engineering was based on the use of differentiated cells of the adult organism [1]. Isolation of such cells was associated with a high risk of invasive and transplantation of pathologically changed cells [2]. Since the identification of stem cells in bone marrow (BMSCs) 40 years ago, they have become a standard tool of regenerative medicine, mainly due to high differentiation potential [4,5]. Liposuction is currently the most commonly performed aesthetic surgery in the world. It is well-tolerated, safe c 2015 The Authors.
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