Abstract
The mechanism by which silver salts interfere with ethylene action in plants is unknown. Prior research indicates that silver acts outside the plasma membrane. We tested a number of plant apoplastic enzymes for inhibition by silver salts. Tomato (Lycopersicon esculentum) fruit pericarp cell wall enzymes, phosphatase, cellulase, peroxidase and polygalacturonase were not inhibited by 100μM AgNO3. At 100μM AgNO3 pectin methyl esterase was inhibited 24.9% and invertase 68.6%; β-galactosidase was inhibited 98.0% by 10μM AgNO3. β-Galactosidase and invertase were not inhibited by silver thiosulfate (STS) at concentrations commonly used to inhibit ethylene action. In other experiments, pea (Pisum sativum) sub-apical hooks were excised and tested in vivo for NADH-ferricyanide oxidoreductase activity. This enzyme was inhibited by 1μM AgNO3 and showed some inhibition by STS. These results indicate that the tomato cell wall enzymes tested are not involved in silver inhibition of plant responses to ethylene. The possible involvement of the oxidoreductase and other plasma membrane enzymes in ethylene action are being considered.
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