Does serotonin-modulating anticonsolidation protein (SMAP) influence the choice of turning direction in carps, Cyprinus carpio, in a T-maze?

Abstract

Serotonin-modulating anticonsolidation protein (SMAP) can impair the formation of memory traces in mammals and fish. We have studied the influence of SMAP on behavioral lateralization of juvenile carps Cyprinus carpio in a T-maze without food reinforcement in three experimental groups (n=8 each): (1) negative control (intact animals); (2) experimental group (fish injected ICV with SMAP; 2μl, 1.2mgml(-1)) and (3) active control group (fish injected ICV with inactivated SMAP). The behavioral lateralization of carps was observed on the 1st, 2nd, 3rd and 6thdays after the injections. In each observation session, a fish was placed five times in a start chamber of the T-maze. The direction of the turn upon leaving the start chamber, as well as the latency from the opening of start chamber flap to the fish's turn was registered. The number of right turns (of all five turns observed during the session) was a criterion of lateralization. It was found that carps have no inherent preference for turning left or right. The SMAP injection did not influence the choice of turning direction, but increases latency values insignificantly. The results are important for the correct interpretation and clarification of data reporting the role of SMAP in training and formation of spatial memory of fish in a maze.