Does physical interstimulus variance account for early electrophysiological face sensitive responses in the human brain? Ten lessons on the N170

Abstract

A recent event-related potential (ERP) study (Thierry G., Martin, C.D., Downing, P., Pegna, A.J. 2007. Controlling for interstimulus perceptual variance abolishes N170 face selectivity. Nature Neuroscience, 10, 505–11) claimed that the larger occipito-temporal N170 response to pictures of faces than other categories – the N170 effect – is due to a methodological artifact in stimulus selection, specifically, a greater interstimulus physical variance between pictures of objects than faces in previous ERP studies which, when controlled, eliminates this N170 effect. This statement casts doubts on the validity of the conclusions reached by a whole tradition of electrophysiological experiments published over the past 15 years and questions the very interest of using the N170 to probe the time course of face processes in the human brain. Here we claim that this physical variance factor is ill-defined by Thierry et al. and cannot account for previous observations of a smaller N170 amplitude to nonface objects than faces without latency increase and component “smearing”. Most importantly, this factor was controlled in previous studies that reported robust N170 effects. We demonstrate that the absence of N170 effect in the study of Thierry et al. is due to methodological flaws in the reported experiments, most notably measuring the N170 at the wrong electrode sites. Moreover, the authors attributed a modulation of N170 amplitude in their study to a differential interstimulus physical variance while it probably reflects a biased comparison of different quality sets of individual images. Here, by taking Thierry et al.’s study as an exemplar case of what should not be done in ERP research of visual categorization processes, we provide clarifications on a number of methodological and theoretical issues about the N170 and its largest amplitude to faces. More generally, we discuss the potential role of differential visual homogeneity of object categories as well as low-level visual properties versus high-level visual processes in accounting for early face-preferential responses and the question of the speed at which visual stimuli are categorized as faces. This survey of the literature points to the N170 as a critical event in the time course of face processes in the human brain.