Does Lactobacillus plantarum-ATCC8014 alleviate inflammation? In vitro and in vivo appraisal

Abstract

ABSTRACT Probiotics are beneficial for certain illnesses, and a variety of clinical studies have reported remarkable effects in the cutaneous system. The objective of current study was to test the antimicrobial impact of Lactobacillus plantarum-ATCC8014 filtrate and lyophilized cells on various pathogenic bacterial strains isolated from skin. The filtrate and lyophilized cell solution of L. plantarum-ATCC8014 showed various impacts on isolated bacteria from the skin. L. plantarum-ATCC8014 filtrate has the highest inhibition zones versus all tested bacteria compared to lyophilized cell solution, whereas it showed the highest action toward S. aureus which had been selected to induce infection, as well as comparing the anti-inflammatory impact of both forms. Five animal groups were tested after the induction of a Staphylococcus aureus wound infection model and monitored for comparative evaluation of the beneficial impact of L. plantarum-prepared forms in the healing process. L. plantarum filtrate has higher antibacterial action compared to lyophilized cells as well as an anti-inflammatory value of 12.7 ± 2.1 µg/ml. Furthermore, L. plantarum filtrate accelerates wound healing, which is indirectly related to animals’ body weight. Microbiological counting of bacterial load in skin and internal organs, including the liver and spleen, revealed that treatment with L. plantarum filtrate led to a notable reduction in bacterial load and prevented its dissemination. Histological examination confirmed the impact of the prepared filter in controlling scars and normalized mast cells, which regulate inflammation. Inflammatory cytokines and oxidative enzyme testing in all groups reflected the protective roles of L. plantarum filtrate with minimal burden on body functions.