Does intracytoplasmic sperm injection effect blastocyst development?

Abstract

ObjectiveThe objective of this study is to determine if intracytoplasmic sperm injection (ICSI) has any detrimental effect on blastocyst development when compared to conventional insemination (CI).DesignRetrospective analysis of in vitro fertilization cycles where half the oocytes were treated with ICSI and half with CI methods.Materials and methodsIn this study all day 5 transfers where half the oocytes were treated with ICSI and half with CI were included. Fertilization and blastocyst development was monitored. Fertilization was described by the total number of oocytes displaying two pronuclei divided by the total number of oocytes inseminated. Rate of blastocyst was calculated where the total blastocysts divided by the total number of day 3 embryos cultured.ResultsA total of 52 retrievals were completed. Half the oocytes were subjected to ICSI and half to CI methods. There were 462 metaphase II oocytes treated with ICSI and 665 oocytes with CI. There was a significantly (P<0.001) higher fertilization rate for ICSI when compared to CI. A total of 365 (79%) fertilized oocytes with ICSI and 342 (51%) with CI. Of the fertilized oocytes 301 ICSI and 292 CI day 3 embryos were cultured to blastocyst. No significant difference was observed for blastocyst development rates between the two insemination methods. There was a total of 137/301 (46%) blastocyst derived from ICSI and 144/292 (49%) for CI fertilization methods.ConclusionInter-patient variation is removed by splitting the insemination methods in the same patient. There have been reports of lower blastocyst development rates with ICSI. However, this study has shown that there were no detrimental effects from ICSI on blastocyst development. ObjectiveThe objective of this study is to determine if intracytoplasmic sperm injection (ICSI) has any detrimental effect on blastocyst development when compared to conventional insemination (CI). The objective of this study is to determine if intracytoplasmic sperm injection (ICSI) has any detrimental effect on blastocyst development when compared to conventional insemination (CI). DesignRetrospective analysis of in vitro fertilization cycles where half the oocytes were treated with ICSI and half with CI methods. Retrospective analysis of in vitro fertilization cycles where half the oocytes were treated with ICSI and half with CI methods. Materials and methodsIn this study all day 5 transfers where half the oocytes were treated with ICSI and half with CI were included. Fertilization and blastocyst development was monitored. Fertilization was described by the total number of oocytes displaying two pronuclei divided by the total number of oocytes inseminated. Rate of blastocyst was calculated where the total blastocysts divided by the total number of day 3 embryos cultured. In this study all day 5 transfers where half the oocytes were treated with ICSI and half with CI were included. Fertilization and blastocyst development was monitored. Fertilization was described by the total number of oocytes displaying two pronuclei divided by the total number of oocytes inseminated. Rate of blastocyst was calculated where the total blastocysts divided by the total number of day 3 embryos cultured. ResultsA total of 52 retrievals were completed. Half the oocytes were subjected to ICSI and half to CI methods. There were 462 metaphase II oocytes treated with ICSI and 665 oocytes with CI. There was a significantly (P<0.001) higher fertilization rate for ICSI when compared to CI. A total of 365 (79%) fertilized oocytes with ICSI and 342 (51%) with CI. Of the fertilized oocytes 301 ICSI and 292 CI day 3 embryos were cultured to blastocyst. No significant difference was observed for blastocyst development rates between the two insemination methods. There was a total of 137/301 (46%) blastocyst derived from ICSI and 144/292 (49%) for CI fertilization methods. A total of 52 retrievals were completed. Half the oocytes were subjected to ICSI and half to CI methods. There were 462 metaphase II oocytes treated with ICSI and 665 oocytes with CI. There was a significantly (P<0.001) higher fertilization rate for ICSI when compared to CI. A total of 365 (79%) fertilized oocytes with ICSI and 342 (51%) with CI. Of the fertilized oocytes 301 ICSI and 292 CI day 3 embryos were cultured to blastocyst. No significant difference was observed for blastocyst development rates between the two insemination methods. There was a total of 137/301 (46%) blastocyst derived from ICSI and 144/292 (49%) for CI fertilization methods. ConclusionInter-patient variation is removed by splitting the insemination methods in the same patient. There have been reports of lower blastocyst development rates with ICSI. However, this study has shown that there were no detrimental effects from ICSI on blastocyst development. Inter-patient variation is removed by splitting the insemination methods in the same patient. There have been reports of lower blastocyst development rates with ICSI. However, this study has shown that there were no detrimental effects from ICSI on blastocyst development.