Abstract
Release of 14CO 2 during metabolism of [1- 14C]glycolate was studied in purified, intact and Triton X-100-solublized peroxisomes isolated from leaves of Secale cereale. Decarboxylation, apparently resulting from H 2O 2 attack on glyoxylate, was stimulated in both intact and solubilized peroxisomal preparations by the catalase inhibitors aminotriazole and sodium azide. CO 2 evolution was also observed in solubilized peroxisomes in the absence of inhibitor when an amino donor was not provided for conversion of glyoxylate to glycine. Loss of CO 2 from labelled glycolate in the absence of amino donors was reduced by addition of exogenous catalase to the reaction medium. Intact peroxisomes showed no glycolate decarboxylation whether or not amino donors were supplied. It appears that the CO 2 release from glycolate observed in previous investigations may be an artifact of the peroxisomal preparations and the assay systems used, and not a significant factor in photorespiratory metabolism in vivo.
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