Does centrifugation and semen processing with swim up at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature?

Abstract

AIM:To evaluate whether semen processing at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature (RT) by swim-up method.SETTINGS:This study was done at tertiary care center attached to Reproductive Medicine Unit and Medical College.DESIGN:Prospective pilot study.PATIENTS:Normozoospermic men (n = 50) undergoing diagnostic semen analysis.MATERIALS AND METHODS:Normozoospermic samples (World Health Organization, 2010 criteria) after analysis was divided into two aliquots (0.5 mL each); one was processed at 37°C and the other at RT by swim-up method. DNA fragmentation of both samples post wash was calculated by acridine orange method.STATISTICAL ANALYSIS USED:The values of sperm DNA fragmentation were represented as mean and standard error (mean ± SEM) of the mean. Paired t-test was used for calculating the sperm DNA integrity difference between post wash at RT and 37°C.RESULTS:Statistically significant difference was not observed in post wash sperm DNA fragmentation values at 37°C compared to RT.CONCLUSION:Our data represents that there was no significant difference in sperm DNA fragmentation values of samples processed at 37°C and at RT. Hence, sperm processing at 37°C does not yield sperm with better DNA integrity compared to centrifugation and processing at RT.