Does a surface coating inhibit bacterial adhesion on silicone hydrogel contact lenses

Abstract

Event Abstract Back to Event Does a surface coating inhibit bacterial adhesion on silicone hydrogel contact lenses Kevin Mccabe1 1 Johnson & Johnson Vision Care inc, Research & Development, United States Purpose: Adhesion of bacteria to contact lens (CL) surfaces is a major contributory factor in the development of CL adverse events. This study sought to determine if surface treating silicone hydrogel (SiHy) CL offers any substantial benefits against adhesion of gram positive and negative bacteria. Method: Two surface-treated SiHy CL (lotrafilcon B; delefilcon A) & 3 uncoated SiHy CL (comfilcon A; samfilcon A; senofilcon A) from manufacturers packaging were soaked in an artificial tear mimicking solution for 15 h under rotative agitation at 35°C. Lenses (n=15) were then rinsed and challenged with either P aeruginosa ATCC 9027 & S. aureus ATCC 25923, using an inoculum of approx. 1x106 colony forming units per milliliter (CFU/mL) for 15 h. Adherent bacteria were recovered and the bacterial supernatant was enumerated for viable microorganisms by a spiral plate technique. CFU counts were determined using an automated colony counter. Results: The 2 surface-treated SiHy CL (lotrafilcon B and delefilcon A) exhibited average Log colony forming units (CFU) of 5.64 & 5.86 for P. aeruginosa and 3.74 & 3.83 for S. aureus respectively. The counts for the 3 uncoated SiHy CL (comfilcon A; samfilcon A; senofilcon A) were 5.86, 5.86 & 5.70 for P. aeruginosa and 3.89, 3.85 & 4.02 for S. aureus. No significant difference was determined between surface-treated and untreated materials (p=0.268). P. aeruginosa exhibited a higher propensity to adhere to the materials than S. aureus (p<0.001). Conclusions: In vitro bacterial adhesion was not modified by applying a surface treatment to the lens materials evaluated and all materials accumulated the gram negative bacteria to a greater extent than the gram positive organism. This suggests that the surface treatments applied to lotrafilcon B and delefilcon A does not provide a protective effect from bacterial adhesion. Further evaluation of the materials post-wear is required to validate the clinical relevance of these in vitro findings. Centre for Contact lens research at the University of Waterloo Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Biomaterials for ophthalmic applications Citation: Mccabe K (2016). Does a surface coating inhibit bacterial adhesion on silicone hydrogel contact lenses. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02416 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Kevin Mccabe Google Kevin Mccabe Google Scholar Kevin Mccabe PubMed Kevin Mccabe Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.