Abstract
Lacidipine (LAC) is a calcium channel blocker used in treatment of hypertension. In this study high performance liquid chromatography method (HPLC) is applied to the determination of LAC in rat plasma. The design of experiment used 3- factor 3- level Box Behnken Design for optimization of mobile phase condition. The effluent was monitored by UV detector at 240 nm, at a flow rate of 1.0 mL/min using amlodipine as internal standard. A linear calibration curve very well fits our data from 20-1200 ng/mL with limit of detection (LOD) quantitation (LOQ) 1.490 and 4.848 ng mL-1 respectively. The method was found to be linear, precise and accurate. Analytes were stable under various conditions (during freeze-thaw, at room temperature, and under deep freeze conditions). The pharmacokinetic study was performed after application of LAC niosomal transgel in rats and results showed enhance bioavailability by 2.57 fold as compared to oral formulation.
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