Abstract
Chronic lymphocytic leukemia is an indolent disorder with an increased infectious risk remaining one of the main causes of death. Development of therapies with higher safety profile is thus a challenging issue. Docosahexaenoic acid (DHA, 22:6) is an omega-3 fatty acid, a natural compound of normal cells, and has been shown to display antitumor potency in cancer. We evaluated the potential in vitro effect of DHA in primary CLL cells. DHA induces high level of in vitro apoptosis compared to oleic acid in a dose-dependent and time-dependent manner. Estimation of IC50 was only of 4.813 µM, which appears lower than those reported in solid cancers. DHA is highly active on CLL cells in vitro. This observation provides a rationale for further studies aiming to understand its mechanisms of action and its potent in vivo activity.
Highlights
Introduction clear cells were separated usingFicoll-Plaque Statistical analysisPLUS (GE Healthcare, Little Chalfont, UK) and purified by magnetic negative immunoselec- Analyses were performed using the Chronic lymphocytic leukemia (CLL) is the tion [B Cell isolation Kit (B-CLL), Miltenyi GraphPad Prism[5] software
B-CLL cells were Statistical significance was defined as P
We evaluated the induction of apoptosis by DHA in CLL cells by studying the effect of various doses and various durations of expo
Summary
A monounsaturated omega-9 FA (OA, 18:1, n-9), was used as a control for the evaluation of the specific effect of DHA. Both OA and DHA were purchased from SigmaAldrich (Saint Louis, MO, USA) and stored after dilution in absolute ethanol at the concentration of 30 mM at −20°C in glass tube to avoid adsorption on plastic. DHA is highly active on CLL cells in vitro. This observation provides a rationale for further studies aiming to understand its mechanisms of action and its potent in vivo activity.
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