Abstract
We investigated the claim that symbiotic dinoflagellates from corals and sea anemones release lipid droplets in vitro. Putative lipid droplet secretion in vitro is taken as evidence for lipid translocation from algae to host in hospite in symbiotic cnidarians. We confirmed that symbiotic algae isolated from the tropical sea anemone Condylactis gigantea exhibited surface “blebs” and attached droplets. The surface entities stained positively with the DNA‐specific fluorochrome Hoechst 33258 and with the chromosome stain lacto‐aceto‐orcein, but not with the lipid lysochrome Sudan Black B. In contrast, bona fide lipid droplets isolated from C. gigantea gonad stained positively with Sudan Black B. The data support the interpretation that droplets attached to isolated symbiotic dinoflagellates are host cell nuclei and are consistent with ultrastructural data published elsewhere.
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