DO PUNICALAGINS HAVE POSSIBLE IMPACT ON SECRETION OF STEROID HORMONES BY PORCINE OVARIAN GRANULOSA CELLS?

Dagmara Packová,Adriana Kolesárová

doi:10.15414/jmbfs.2016.5.special1.57-59

Dagmara Packová, Adriana Kolesárová

Open Access

https://doi.org/10.15414/jmbfs.2016.5.special1.57-59

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Abstract

Punicalagin is a major component responsible for pomegranate's antioxidant properties. Punicalagin is the predominant ellagitannin of Punica granatum and present in two isomeric forms; punicalagin α and β. Punicalagins are metabolised to ellagic acid (antioxidant) and microorganisms present in colon can metabolize ellagic acid to urolithins – each other substances could be responsible for effect on cell intracellular mechanism. The aim of our study was to observe the effect of punicalagins on secretion of steroids hormones – progesterone and 17β-estradiol. Ovarian granulosa cells were cultivated during 24h without (control group) and with various doses (0.01, 0.1, 1, 10 and 100 µg.ml-1) of pomegranate compounds – punicalagins. Steroid hormones progesterone and 17β-estradiol were evaluated by ELISA. Obtained results showed that the secretion of progesterone and 17β-estradiol by ovarian granulosa cells was not significantly (P≥0.05) influenced by punicalagins. In this pilot study no effects of punicalagins on porcine ovarian granulosa cells were found.

Highlights

Isolation of granulosa cellsPunicalagins are ellagitannins in which gallagic and ellagic acid are linked to a glucose molecule (Cerdá et al 2003)
Punicalagins were metabolized and/or absorbed. This means that most of the ingested punicalagins have to be transformed to known or unknown metabolites or accumulated in tissue (Cerdá et al 2003). This in vitro study was focused on the secretion of steroid hormones, progesterone and 17β-estradiol, by porcine ovarian granulosa cells after punicalagin administration
Nagata et al (2007) have shown that pomegranate juice is a potent inhibitor of CYP2C9 and CYP3A enzymes – these enzymes belong to cytochrome p450 group; and are responsible for cholesterol, steroid and lipid metabolism or synthesis

Summary

Introduction

Punicalagins are ellagitannins in which gallagic and ellagic acid are linked to a glucose molecule (Cerdá et al 2003). This means that most of the ingested punicalagins have to be transformed to known (punicalin, ellagic acid, gallagic acid) or unknown metabolites or accumulated in tissue (Cerdá et al 2003). This in vitro study was focused on the secretion of steroid hormones, progesterone and 17β-estradiol, by porcine ovarian granulosa cells after punicalagin administration

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