Do gene expression changes in articular cartilage proteases of the synovial membrane correlate with expression changes of the same genes in systemic blood cells?

Adam Kwapisz,Marcin Domżalski,Marek Synder,Michał Chojnacki,Andrzej Grzegorzewski

doi:10.1007/s00264-013-2195-8

Adam Kwapisz, Marcin Domżalski + Show 3 more

Open Access

https://doi.org/10.1007/s00264-013-2195-8

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Journal: International Orthopaedics	Publication Date: Nov 22, 2013
Citations: 27	License type: CC BY 2.0

Affiliation: Medical University of Lodz

Abstract

PurposeThe aim of our study was to find whether an injury of the knee joint tissues increases gene expression of selected hyaline cartilage degenerating enzymes such as matrix metaloproteinases (MMP) and aggreacaneses (Agg).MethodsA total of 138 patients (81 female, 57 male) were admitted for knee arthroscopy with a mean age of 38.8 years. Full blood samples were collected preoperatively and synovium samples intraoperatively. Joint tissue lesions such as menisci, anterior cruciate ligament (ACL) and hyaline cartilage were estimated. Real time PCR with spectrophotometric analysis was performed.ResultsAn ACL lesion was found in 56 patients, medial menisci (MM) in 65, and lateral menisci (LM) in five. Chondral lesions were estimated according to Outerbridge’s grading system. In laboratory tests correlation between ACL tear and gene expression was seen except TIMP1 in serum (p < 0.05). In MM lesions MMP9, Agg2 elevation in serum was observed. LM lesions erased MMP13, MMP14 in serum and MMP8 in synovium. Chondral lesions revealed that many genes had higher expression in patients without hyaline degeneration. All of the gene expressions correlated between serum and synovium.ConclusionAn ACL lesion provokes elevation in expression of proteases genes, while the influence of other lesions remains elusive. Gene expression in synovium correlates with peripheral blood.

Highlights

It is estimated that, during the year 1995, 40 million people were treated for osteoarthritis (OA) in the United States of America, with a simultaneous prognosis of the annual incidence of the disease to rise up to 59.4 patients in 2020 [1]
Osteoarthritis is associated with some prevalence of catabolic processes of the hyaline cartilage vs. its regenerative processes [5, 6]
Among the above-mentioned enzymes, the key role in articular cartilage destruction is played by adamlysins and matrix metalloproteinases (MMPs)[9, 10]

Summary

Introduction

It is estimated that, during the year 1995, 40 million people were treated for osteoarthritis (OA) in the United States of America, with a simultaneous prognosis of the annual incidence of the disease to rise up to 59.4 patients in 2020 [1]. Gelber et al demonstrated that, among subjects with a history of knee joint trauma, as many as 13.9 % of them presented with OA before the 65th year of life, while it was only 6 % in the control group [3]. In the Framingham study, a five-fold higher risk of developing OA was estimated in subjects after knee joint trauma in history [4]. Osteoarthritis is associated with some prevalence of catabolic processes of the hyaline cartilage vs its regenerative processes [5, 6] These changes are controlled by inflammatory cytokines from the synovial membrane and chondrocytes, such as, for example, IL1 and TNF-α. Among the above-mentioned enzymes, the key role in articular cartilage destruction is played by adamlysins (including aggrecanases [Agg]) and matrix metalloproteinases (MMPs)[9, 10]

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Conclusion