Abstract
The aim of this study was to evaluate the antimicrobial action of different endodontic pastes against Enterococcus faecalis ATCC 29212, isolated from the urinary tract, and compare the action with E. faecalis ATCC 4083, isolated from the root canal. For this purpose, dentin blocks were infected for 21 days with both bacteria at different time-intervals to ensure there would be no cross contamination. After this period, blocks were immersed in the test medications for 7 days, according to the following groups: CH/S, CH/P, CH/CMCP, CH/CHX, CH/DAP and TAP. Images of the samples were captured with a confocal microscope and the percentage of live cells was computed by means of the Bioimage program. The ATCC 29212 strain was shown to be more resistant to CH/SS, Calen, CH/DAP, and TAP than the ATCC 4083 strain. The antimicrobial action of the medications against each strain were divergent concerning the order of susceptibility. The authors concluded that the strains behaved in a different manner: in general, those extracted from the urinary tract were more resistant to the tested medications. Therefore, when E. faecalis must be used for in vitro research in endodontics, we suggest the use of ATCC 4083 strain to obtain results that are closer to the clinical reality.
Highlights
Submitted: Aug 23, 2017 Accepted for publication: Mar 15, 2018 Last revision: Apr 13, 2018Inflammatory lesions of the peri-radicular tissues result from an infection caused by the aggressive invasion of microorganisms from the root canal system that resisted the antibodies and immune cells of the host.[1]
Enterococcus faecalis are the bacteria most frequently found in root canals of re-treatment and persistent infection cases, since they are capable of adapting to adverse environments and have a high level of pathogenicity that leads to perpetuation of the endodontic infection.[2]
Differences were observed in the susceptibility of E. faecalis ATCC 4083 and ATCC 29212 to the studied pastes; b
Summary
Inflammatory lesions of the peri-radicular tissues result from an infection caused by the aggressive invasion of microorganisms from the root canal system that resisted the antibodies and immune cells of the host.[1] Enterococcus faecalis are the bacteria most frequently found in root canals of re-treatment and persistent infection cases, since they are capable of adapting to adverse environments and have a high level of pathogenicity that leads to perpetuation of the endodontic infection.[2]. The application of intracanal medications is one of the strategies used for root canal antisepsis and the most frequently used medication is calcium hydroxide (CH), which releases hydroxyl ions in an aqueous solution, providing a highly alkaline environment that affects most microorganisms in infected root canals. Microorganisms resistant to CH such as E. faecalis,[3] Candida species,[4] and Actinomyces radicidentis[5] have been associated with endodontic failure
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