Abstract
Recently, many culinary mushrooms have become attractive sources of biologically but non-pharmacological activities including immune modulating activities. In this study, the fibrinolytic activities of freeze-dried basidiocarps of ten edible mushrooms were determined using a qualitative fibrin plate assay and a quantitative enzyme assay using spectrophotometric method. Further, the crude extracts of all the ten mushrooms were subjected to an aqueous two-phase system (ATPS) to recover the fibrinolytic enzymes. The crude extracts and ATPS extracts of Lentinula edodes showed the highest fibrinolytic activity of 48.06 U/mg and 54.28 U/mg, respectively assessed via the Folin-spectrophotometric method. The recovery of fibrinolytic enzymes from L. edodes was the highest and the fibrinolytic enzymes were further analysed through gel electrophoresis study. A 50 kDa sized fibrinolytic enzyme from L. edodes was revealed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Biomed Rev 2017; 28:91-99. Keywords: edible mushroom, fibrinolytic enzyme, Lentinula edodes, aqueous two-phase system
Highlights
E-mail: Ali, Tan, Raman, Lakshmanan, Ling, Phan, and Sabaratnam health and strengthen the immune system, which have becomplex from Flammulina velutipes by gel chromatography ratio
The polyethylene glycol (PEG) and the phosphate based Aqueous two-phase system (ATPS) were used based on a previous
The top layer was carefully pipetted by a micropipette in each system and used as the ATPS recovtivity of the crude mushroom extracts and aqueous two-phase calculated according to Eq 1: spectrophotometric method
Summary
Pers., Pleurotus cystidiosus O.K. Mill and Grifola frondosa tracts Aqueous two-phase system (ATPS) extraction (ATPS recovered samples). (crude extracts and ATPS recovered samples) were carefully replaced by 0.2 mL of distilled water and mixed with 5 mL of Coomassie Brilliant Blue reagent for the blank. A lytic zone was Sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) The molecular weight of crude extracts and ATPS recovered diameter measured was directly proportional to the strength
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