Abstract
The possibility of detecting organomercury fungicides by means of thin-layer chromatographic-enzymatic and chemical techniques has been studied. Urease proved to be very sensitive for the detection of mercury compounds and their impurities of which up to five were found in the fungicides studied. The detection limits for the main compounds ranged from 50 ng to i to μg, and for the impurities from i to 60 μg. Bovine liver esterase, α-, and β-amylase are also inhibited by the fungicides and can be used for their detection, but are generally less sensitive. The chemical detection with sodium sulphide and dithizone ranged from 0.5 to 20 μg and the impurities did not interfere with the detection. Two solvent systems, isopropyl ether and chloroform-ethyl acetate (10:4), respectively, were suitable for the separation of nine mercury fungicides.
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