Dünnschichtchromatographische trennung von porphyrinen, hämin und lipoiden auf Kieselgel-H-platten zur bestimmung der erythrozytenporphyrine als methylester

Abstract

Thin-layer chromatographic separation of porphyrins, haemin and lipids on Silica Gel H plates and determination of the erythrocyte porphyrins as their methyl esters The porphyrins of lyophilized erythrocytes were extracted with chloroform after transformation into their methyl esters with methanol–sulphuric acid. The components of the chloroform extract were separated by thin-layer chromatography on 2 mm thick Silica Gel H plates. The porphyrin methyl ester could be completely separated from the protohaemin methyl ester and the lipids contained in the chloroform extract. During the esterification of porphyrins and protohaemin the fatty acids of glycerophosphatides of erythrocytes were transesterified to methyl esters. This was demonstrated by gas-chromatographic analysis. Erythrocyte proto- and coproporphyrin methyl esters were isolated chromatographically and determined by recording spectrophotometry with their absorption maxima.