DNA–cyclodextrin–inorganic hybrid material for absorbent of various harmful compounds

Abstract

A DNA–cyclodextrin (CD)–inorganic hybrid material was prepared by mixing the DNA, CD, and silane coupling reagents, such as 3-aminopropyltriethoxysilane (APTES) or tetraethoxysilane (TEOS). The DNA–PCD–APTES hybrid material possessed a high water-stability. Additionally, the tensile strength of the hybrid material containing the APTES was approximately two times higher than that of hybrid material without the inorganic component. These chemical and physical properties were due to the formation of a siloxane network in the hybrid material and the electrostatic interaction between the phosphate group in the DNA and amino group in the APTES. Furthermore, the DNA–PCD–APTES hybrid material could be immobilized on a glass filter by the coupling reaction to the silanol group in the filter. When an aqueous multi-component solution, which contained dibenzofuran, biphenyl, bisphenol A, diethylstilbestrol, and naphthalene, was applied to DNA–PCD–APTES hybrid filter, this filter could effectively accumulate various harmful compounds, such as dioxin, polychlorobiphenyl (PCB), and bisphenol A. Especially, the accumulation of diethylstilbestrol was extremely high and this value was approximately 85%. In contrast, DNA–PCD–TEOS hybrid, that was prepared by the mixing of TEOS without the amino groups, was a fragile material and could not use for the adsorbent of harmful compounds.