Abstract
Lactic acid bacteria commonly named as probiotics have a broad range of utilization area in human diet and food industry. Besides these known properties of probiotics, in recent years many researchers have focused on their anti-genotoxic called DNA-bioprotective effects. Human gets exposure with genotoxins such as mycotoxins, polycyclic aromatic hydrocarbons, and n-nitroso-compounds through diet and environmental contaminations. Aflatoxin B1 is mycotoxin which was reported one of the most potent hepato-carcinogen and its exposure stems from human diet. In this study, we aimed to investigate DNA-bioprotective effect of Lactobacillus plantarum on human colon adenocarcinoma (Caco-2) cells against Aflatoxin B1 (AFB1) with comet assay without metabolic activation. The results showed that DNA-bioprotective effect of L. plantarum did reduce the AFB1’s genotoxic effect on colon adenocarcinoma (Caco-2) cells. Positive control (50 μM H2O2 applied) and high dose (>10ppm) AFB1 applied cells have the same comet tail appearance. Only visual scoring is performed. Besides negative control cells (Only PBS) and Lactobacillus plantarum+AFB1 mixture showed the same manner with each other; no comet tail detected. These results clearly indicate that L. plantarum is capable of reduce AFB1 safely without producing any by-products.
Highlights
Lactic acid bacteria are a wide microorganism group which is known for formation of lactic acid as a dominant metabolite with their sugar metabolism (Zhong et al 2014)
Lactic acid bacteria commonly named as probiotics have a broad range of utilization area in human diet and food industry
In this study we aimed to investigate DNAbioprotective effect of Lactobacillus plantarum on human colon adenocarcinoma (Caco-2) enterocytes against Aflatoxin B1 (AFB1) with comet assay without metabolic activation
Summary
Lactic acid bacteria are a wide microorganism group which is known for formation of lactic acid as a dominant metabolite with their sugar metabolism (Zhong et al 2014). Lactic acid fermentation is the most common and the oldest way of food preservation. Besides preservation aspects of lactic acid bacteria are seen from mouth to the end of the intestinal system of human and other mammals (Molin 2001, de Vries et al 2006). Lactic acid bacteria commonly named as probiotics have a broad range of utilization area in human diet and food industry. Probiotics are defined as “living microorganisms when consumed adequate amounts provide health benefit to the host” by FAO/WHO working group (FAO/WHO, 2002). The use of probiotics has an increasing demand for their prevention and treatment of diseases mainly intestinal diseases (Zhong et al 2014). In recent years many researchers have focused on their anti-genotoxic called DNAbioprotective effects
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
