水溶性碳纳米粒子的制备及其在DNA和单核苷酸多态性分析中的应用

Abstract

Water-soluble carbon nanoparticles (CNPs) with a diameter of 7-12 nm were prepared with electrochemical oxidation. The CNPs exhibit high efficiency to quench the fluorescence of fluorescently labeled ssDNA probes by adsorbing the ssDNA on the CNPs surface through π-π interaction. The fluorescence can be recovered by addition of target DNA complementary to the ssDNA probe through hybridization to form double-strand DNA (dsDNA). Therefore, the CNPs offer an effective analysis platform for detection of DNA, in which 1-200 nmol/L target DNA can be detected. Moreover, in the presence of CNPs, the melting temperature (<italic>T</italic>_m) of dsDNA containing fluorescently labeled ssDNA and DNA target can be determined, when the dsDNA contains mismatched bases, the <italic>T</italic>_m decreases. Based on the differentiation <italic>T</italic>_m values, the CNPs-based assay can be conveniently applied to analyze single nucleotide polymorphisms rapidly.