Abstract
30Jun 2016 DNA Yield and Concentration Modeling by Age, A260/280 and A260/230 Ratios Using Multiple Linear regression (MLR), Bootstrap and Response Surface Methodology (RSM). Siti Nazihahasma Hassan , Wan Suriana Wan Ab Rahman , Wan Muhamad Amir W Ahmad , Suharni Mohamad , Rosline Hassan and Selamah Ghazali. School of Dental Sciences. School of Medical Sciences.
Highlights
Epidemiologists are increasingly trying to supplement observational data with biological material, including deoxyribonucleic acid (DNA)
The purity of the extracted DNA is usually confirmed by the UV spectrophotometer and calculated as the 260/280 OD ratio and 260/230 OD ratio
Falsely elevated A260/A280 and A260/A230 purity ratios could be resulted from buffer salts, such as Trisaminomethane (Tris), ethylenediaminetetraacetic acid (EDTA), and guanidine isothiocyanate contaminations (Wilfinger et al, 1997)
Summary
DNA Yield and Concentration Modeling by Age, A260/280 and A260/230 Ratios Using Multiple Linear regression (MLR), Bootstrap and Response Surface Methodology (RSM). Siti Nazihahasma Hassan[1], Wan Suriana Wan Ab Rahman1*, Wan Muhamad Amir W Ahmad[1], Suharni Mohamad[1], Rosline Hassan[2] and Selamah Ghazali[2]. 1. School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan. 2. School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan
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