DNA vaccine encoding hepatitis C virus proteins NS3-NS5B induces more effective immune response in comparison with DNAs expressing individual nonstructural genes

Abstract

Event Abstract Back to Event DNA vaccine encoding hepatitis C virus proteins NS3-NS5B induces more effective immune response in comparison with DNAs expressing individual nonstructural genes Olga V. Masalova1*, Ekaterina I. Lesnova1, Alexander V. Ivanov2, Alexey V. Pichugin3, Kristina Y. Permyakova1, Ravshan I. Ataullakhanov3 and Alla A. Kushch1 1 D.I.Ivanovsky Institute of Virology, Russian Ministry of Health, Russia 2 Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Russia 3 Institute of Immunology, Russian Ministry of Health, Russia Currently, there are several DNA vaccines against hepatitis С virus (HCV) being tested, but the optimal composition of HCV genes is not yet defined. The goal of study was to evaluate the effectiveness of genetic immunization of mice with DNA pcNS3-NS5B encoding five hepatitis C virus (HCV) nonstructural proteins: NS3, NS4A, NS4B, NS5A and NS5B in comparison with plasmids containing genes of the same individual nonstructural proteins. The HCV DNA constructions with gene GM-CSF as adjuvant were injected intramuscularly in DBA mice three times. Humoral immune response was assessed with ELISA; cellular immune response - in blast transformation reaction, by quantitation of CD4+ and CD8+ T cell proliferation using flow cytofluorimetry, by intracellular synthesis and secretion of IFN-γ and IL-2 in ELISpot and ELISA. It was found that the functionally active T cell response was achieved to antigens presenting NS3, NS4, NS5A and NS5B epitopes of different HCV genotypes in response to pcNS3-NS5B plasmid and was stronger than that to plasmids carrying individual genes. Injection of pcNS3-NS5B induced high CD4+ Т cells proliferation, IL-2 and IFN-γ secretion, anti-NS3 and anti-NS5B IgG2a production. These findings indicate that genetic immunization with pcNS3-NS5B is promising approach for development of hepatitis C vaccine. Keywords: hepatitis C virus (HCV), nonstructural proteins, DNA immunization, immune response, gene GM-CSF Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Masalova OV, Lesnova EI, Ivanov AV, Pichugin AV, Permyakova KY, Ataullakhanov RI and Kushch AA (2013). DNA vaccine encoding hepatitis C virus proteins NS3-NS5B induces more effective immune response in comparison with DNAs expressing individual nonstructural genes. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00162 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Olga V Masalova, D.I.Ivanovsky Institute of Virology, Russian Ministry of Health, Moscow, Russia, ol.mas@mail.ru Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Olga V Masalova Ekaterina I Lesnova Alexander V Ivanov Alexey V Pichugin Kristina Y Permyakova Ravshan I Ataullakhanov Alla A Kushch Google Olga V Masalova Ekaterina I Lesnova Alexander V Ivanov Alexey V Pichugin Kristina Y Permyakova Ravshan I Ataullakhanov Alla A Kushch Google Scholar Olga V Masalova Ekaterina I Lesnova Alexander V Ivanov Alexey V Pichugin Kristina Y Permyakova Ravshan I Ataullakhanov Alla A Kushch PubMed Olga V Masalova Ekaterina I Lesnova Alexander V Ivanov Alexey V Pichugin Kristina Y Permyakova Ravshan I Ataullakhanov Alla A Kushch Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.