Abstract

In 1990 in Griswold, Connecticut, archaeologists excavated a burial found in a “skull and crossbones” orientation. The lid of the 19th century coffin had brass tacks that spelled “JB55”, the initials of the person lying there and age at death. JB55 had evidence of chronic pulmonary infection, perhaps tuberculosis. It is possible that JB55 was deemed a vampire due to his disease, and therefore had to be “killed” by mutilating his corpse. In an attempt to reveal the identity of JB55, DNA testing was performed. Ancestry informative single nucleotide polymorphism (SNP) analysis using the Precision ID Ancestry Panel indicated European ancestry. A full Y-chromosomal short tandem repeat (Y-STR) profile was obtained, belonging to haplogroup R1b. When the Y-STR profile was searched in the publicly accessible FamilyTreeDNA R1b Project website, the two closest matches had the surname “Barber”. A search of historical records led to a death notice mentioning John Barber, whose son Nathan Barber was buried in Griswold in 1826. The description of Nathan Barber closely fits the burial of “NB13,” found near JB55. By applying modern forensic DNA tools to a historical mystery, the identity of JB55 as John Barber, the 19th century Connecticut vampire, has been revealed.

Highlights

  • In 1990, an unmarked cemetery dating to the 18th–19th centuries was excavated in Griswold, Connecticut, when skeletal remains were encountered during sand and gravel operations [1]

  • The femoral sample of JB55 produced sufficient DNA for single nucleotide polymorphism (SNP) and Y-chromosomal short tandem repeat (Y-short tandem repeats (STRs)) typing based on the Plexor HY quantification (Table 1)

  • The JB55 DNA samples were of relatively high quality, given the age of this historical archaeology case dating to the mid-nineteenth century

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Summary

Introduction

In 1990, an unmarked cemetery dating to the 18th–19th centuries was excavated in Griswold, Connecticut, when skeletal remains were encountered during sand and gravel operations [1]. Genes 2019, 10, 636 twenty-seven burials discovered, a stone-lined grave containing a middle-aged male proved to be veryGenes interesting. Brass tacks on the coffin lid spelled “JB55”, likely indicating the initials 2019, 10, x FOR. REVIEW of 10of the twenty-seven burialsPEER discovered, a stone-lined grave containing a middle-aged male proved to be very deceased and the age at death of 55 years. The remaining hardware included screws and copper interesting. Brass tacks on the coffin lid spelled “JB55”, likely indicating the initials of the deceased and twenty-seven burials discovered, a stone-lined grave containing a middle-aged proved be and dowel hinges, which dated the coffin to the early nineteenth century [1]. The on remaining hardware included and copper dowel hinges, veryat the and coffin lid spelled “JB55”,screws likely(Figure indicating the initials of thewhich femora ofinteresting

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