DNA testing of allelic polymorphism of IGF-1, GDF 5, GH, GHR genes in young beef cattle

Abstract

The development and improvement of methodologies that allow assessing the features of the gene pool of domestic, as well as local breeds, farm animals by breeding-significant characteristics for associative research is of both fundamental and applied importance. To carry out breeding work with cattle to increase meat productivity (breeding and commodity farms), it is advisable to consider DNA genotyping as an additional criterion for breeding and selection of animals. In this study, using PCR-PDRF analysis, the frequency of occurrence of the allele spectrum of genes was determined: insulin-like growth factor-1 (IGF?1), differentiating growth factor (GDF5), somatotropin (GH ? growth hormone), growth hormone receptor (GHR), in order to study the breed and population characteristics of repair young cattle cattle of different breeds: Hereford and Kazakh white-headed, bred in farms of the Stavropol Territory. In the course of the study, complex genotypes were identified, the allelic profile of which is represented by eight and seven desirable alleles of four genes in replacement young animals of the Kazakh white-headed breed and six marker alleles of three and four genes in the Hereford breed. It was also noted that the heterozygosity test in the two studied samples of animals for all genes was negative and ranged from -0.10 to -0.44.