Abstract
1. 1. A method is described for the isolation of DNA from housefly ovaries where small quantities of the nucleic acid are present. 2. 2. Contamination of DNA by residual protein was reduced by treatment with proase. All the DNA samples were essentially free of RNA, as indicated by the orcinol reaction. 3. 3. The average molar base composition (40 per cent of guanine+cytosine) corresponds to Drosophila DNA, and the purine to pyrimidine ratio is close to unity, as would be expected for double stranded DNA. 4. 4. The conversion of (2- 14C) glycine to purines of DNA was used as a measure of the de novo biosynthetic route. Analysis of the DNA, after hydrolysis, demonstrated that the label is present in the purine and pyrimidine bases. 5. 5. The specific activity is highest in the bases of DNA extracted from ovaries from 24-hr-old flies. The decrease in specific activity thereafter is due to the diluting effects in the de novo synthesis.
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